Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1217952..1218590 Replicon chromosome
Accession NZ_CP063467
Organism Escherichia coli strain EM20-18-50

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IQS25_RS05825 Protein ID WP_000813794.1
Coordinates 1218414..1218590 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IQS25_RS05820 Protein ID WP_001270286.1
Coordinates 1217952..1218368 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS25_RS05800 1213104..1214045 - 942 WP_001251304.1 ABC transporter permease -
IQS25_RS05805 1214046..1215059 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
IQS25_RS05810 1215077..1216222 - 1146 WP_123873092.1 ABC transporter substrate-binding protein -
IQS25_RS05815 1216467..1217873 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
IQS25_RS05820 1217952..1218368 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IQS25_RS05825 1218414..1218590 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IQS25_RS05830 1218812..1219042 + 231 WP_000494244.1 YncJ family protein -
IQS25_RS05835 1219134..1221095 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IQS25_RS05840 1221168..1221704 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IQS25_RS05845 1221757..1222971 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1223011..1224159 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T178318 WP_000813794.1 NZ_CP063467:c1218590-1218414 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T178318 NZ_CP083932:c5260416-5260033 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT178318 WP_001270286.1 NZ_CP063467:c1218368-1217952 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT178318 NZ_CP083932:c5260658-5260416 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References