Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2129661..2130299 Replicon chromosome
Accession NZ_CP086336
Organism Escherichia coli strain EBJ001

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag C5N11_RS10295 Protein ID WP_001447010.1
Coordinates 2129661..2129837 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C5N11_RS10300 Protein ID WP_001270286.1
Coordinates 2129883..2130299 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N11_RS10275 (2125280) 2125280..2126455 - 1176 WP_001236265.1 BenE family transporter YdcO -
C5N11_RS10280 (2126547) 2126547..2127083 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C5N11_RS10285 (2127156) 2127156..2129117 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C5N11_RS10290 (2129209) 2129209..2129439 - 231 WP_000494244.1 YncJ family protein -
C5N11_RS10295 (2129661) 2129661..2129837 + 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C5N11_RS10300 (2129883) 2129883..2130299 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C5N11_RS10305 (2130378) 2130378..2131784 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
C5N11_RS10310 (2132029) 2132029..2133174 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
C5N11_RS10315 (2133192) 2133192..2134205 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C5N11_RS10320 (2134206) 2134206..2135147 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2124092..2125240 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T223012 WP_001447010.1 NZ_CP086336:2129661-2129837 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T223012 NZ_CP118275:802360-802770 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT223012 WP_001270286.1 NZ_CP086336:2129883-2130299 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT223012 NZ_CP118275:802113-802379 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References