Detailed information of TA system TA223012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2129661..2130299
• Replicon: chromosome
• Accession: NZ_CP086336
• Organism: Escherichia coli strain EBJ001

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: C5N11_RS10295
• Protein ID: WP_001447010.1
• Coordinates: 2129661..2129837 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C5N11_RS10300
• Protein ID: WP_001270286.1
• Coordinates: 2129883..2130299 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N11_RS10275 (2125280)	2125280..2126455	-	1176	WP_001236265.1	BenE family transporter YdcO	-
C5N11_RS10280 (2126547)	2126547..2127083	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C5N11_RS10285 (2127156)	2127156..2129117	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C5N11_RS10290 (2129209)	2129209..2129439	-	231	WP_000494244.1	YncJ family protein	-
C5N11_RS10295 (2129661)	2129661..2129837	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C5N11_RS10300 (2129883)	2129883..2130299	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C5N11_RS10305 (2130378)	2130378..2131784	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
C5N11_RS10310 (2132029)	2132029..2133174	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
C5N11_RS10315 (2133192)	2133192..2134205	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C5N11_RS10320 (2134206)	2134206..2135147	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.008	flank	IS/Tn	-	-	2124092..2125240	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T223012 WP_001447010.1 NZ_CP086336:2129661-2129837 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T223012 NZ_CP118275:802360-802770 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT223012 WP_001270286.1 NZ_CP086336:2129883-2130299 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT223012 NZ_CP118275:802113-802379 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8