Detailed information of TA system TA143220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1432515..1433153
• Replicon: chromosome
• Accession: NZ_CP045977
• Organism: Escherichia coli strain INSC1002

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GI529_RS07050
• Protein ID: WP_000813794.1
• Coordinates: 1432515..1432691 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GI529_RS07055
• Protein ID: WP_001270286.1
• Coordinates: 1432737..1433153 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GI529_RS07030	1428134..1429348	-	1215	WP_001326689.1	BenE family transporter YdcO	-
GI529_RS07035	1429401..1429937	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
GI529_RS07040	1430010..1431971	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GI529_RS07045	1432063..1432293	-	231	WP_000494244.1	YncJ family protein	-
GI529_RS07050	1432515..1432691	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GI529_RS07055	1432737..1433153	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GI529_RS07060	1433232..1434638	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
GI529_RS07065	1434883..1436028	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
GI529_RS07070	1436046..1437059	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
GI529_RS07075	1437060..1438001	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.007	flank	IS/Tn	-	-	1426946..1428094	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T143220 WP_000813794.1 NZ_CP045977:1432515-1432691 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T143220 NZ_CP060974:c605402-604938 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT143220 WP_001270286.1 NZ_CP045977:1432737-1433153 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT143220 NZ_CP060974:c605737-605402 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 6HPB