Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2362004..2362642 Replicon chromosome
Accession NZ_AP025205
Organism Escherichia coli strain 2017.04.03CC

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LPH27_RS11340 Protein ID WP_000813794.1
Coordinates 2362466..2362642 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LPH27_RS11335 Protein ID WP_001270286.1
Coordinates 2362004..2362420 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH27_RS11315 (2357156) 2357156..2358097 - 942 WP_001251319.1 ABC transporter permease -
LPH27_RS11320 (2358098) 2358098..2359111 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
LPH27_RS11325 (2359129) 2359129..2360274 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
LPH27_RS11330 (2360519) 2360519..2361925 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LPH27_RS11335 (2362004) 2362004..2362420 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LPH27_RS11340 (2362466) 2362466..2362642 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LPH27_RS11345 (2362864) 2362864..2363094 + 231 WP_000494244.1 YncJ family protein -
LPH27_RS11350 (2363186) 2363186..2365147 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LPH27_RS11355 (2365220) 2365220..2365756 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LPH27_RS11360 (2365848) 2365848..2367023 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2367063..2368211 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T40081 WP_000813794.1 NZ_AP025205:c2362642-2362466 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T40081 NZ_AP025205:c2362642-2362466 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT40081 WP_001270286.1 NZ_AP025205:c2362420-2362004 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT40081 NZ_AP025205:c2362420-2362004 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References