Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2499358..2499996 Replicon chromosome
Accession NZ_CP104123
Organism Escherichia coli strain USDA-ARS-USMARC-49610

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag N1705_RS12035 Protein ID WP_000813794.1
Coordinates 2499820..2499996 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag N1705_RS12030 Protein ID WP_001270286.1
Coordinates 2499358..2499774 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1705_RS12010 (2494510) 2494510..2495451 - 942 WP_001251313.1 ABC transporter permease -
N1705_RS12015 (2495452) 2495452..2496465 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
N1705_RS12020 (2496483) 2496483..2497628 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
N1705_RS12025 (2497873) 2497873..2499279 - 1407 WP_219411894.1 PLP-dependent aminotransferase family protein -
N1705_RS12030 (2499358) 2499358..2499774 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
N1705_RS12035 (2499820) 2499820..2499996 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
N1705_RS12040 (2500218) 2500218..2500448 + 231 WP_000494244.1 YncJ family protein -
N1705_RS12045 (2500540) 2500540..2502501 - 1962 WP_147699513.1 23S rRNA 5-hydroxycytidine C2501 synthase -
N1705_RS12050 (2502574) 2502574..2503110 - 537 WP_219411944.1 DNA-binding transcriptional regulator SutR -
N1705_RS12055 (2503163) 2503163..2504377 + 1215 WP_260248478.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2504417..2505565 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T257277 WP_000813794.1 NZ_CP104123:c2499996-2499820 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT257277 WP_001270286.1 NZ_CP104123:c2499774-2499358 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References