Detailed information of TA system TA105805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3239238..3239876
• Replicon: chromosome
• Accession: NZ_CP029574
• Organism: Escherichia coli strain DA33133

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: DLJ62_RS16750
• Protein ID: WP_001447010.1
• Coordinates: 3239238..3239414 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DLJ62_RS16755
• Protein ID: WP_001270286.1
• Coordinates: 3239460..3239876 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ62_RS16730	3234857..3236071	-	1215	WP_001326689.1	BenE family transporter YdcO	-
DLJ62_RS16735	3236124..3236660	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
DLJ62_RS16740	3236733..3238694	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
DLJ62_RS16745	3238786..3239016	-	231	WP_000494244.1	YncJ family protein	-
DLJ62_RS16750	3239238..3239414	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
DLJ62_RS16755	3239460..3239876	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
DLJ62_RS16760	3239955..3241361	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
DLJ62_RS16765	3241606..3242751	+	1146	WP_000047428.1	ABC transporter substrate-binding protein	-
DLJ62_RS16770	3242769..3243782	+	1014	WP_000220395.1	ABC transporter ATP-binding protein	-
DLJ62_RS16775	3243783..3244724	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.008	flank	IS/Tn	-	-	3233669..3234817	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T105805 WP_001447010.1 NZ_CP029574:3239238-3239414 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T105805 NZ_CP029574:3239238-3239414 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT105805 WP_001270286.1 NZ_CP029574:3239460-3239876 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT105805 NZ_CP029574:3239460-3239876 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8