Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 964252..964890 Replicon chromosome
Accession NZ_CP024855
Organism Escherichia coli strain AR_0011

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag AM338_RS05105 Protein ID WP_001447010.1
Coordinates 964252..964428 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AM338_RS05110 Protein ID WP_001270286.1
Coordinates 964474..964890 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM338_RS05085 959871..961085 - 1215 WP_001326689.1 BenE family transporter YdcO -
AM338_RS05090 961138..961674 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AM338_RS05095 961747..963708 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AM338_RS05100 963800..964030 - 231 WP_000494244.1 YncJ family protein -
AM338_RS05105 964252..964428 + 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AM338_RS05110 964474..964890 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AM338_RS05115 964969..966375 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AM338_RS05120 966620..967765 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AM338_RS05125 967783..968796 + 1014 WP_100183743.1 ABC transporter ATP-binding protein -
AM338_RS05130 968797..969738 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 958683..959831 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T88865 WP_001447010.1 NZ_CP024855:964252-964428 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T88865 NZ_CP024855:964252-964428 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT88865 WP_001270286.1 NZ_CP024855:964474-964890 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT88865 NZ_CP024855:964474-964890 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References