Detailed information of TA system TA104651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1090764..1091402
• Replicon: chromosome
• Accession: NZ_CP029057
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FORC81_RS05350
• Protein ID: WP_000813794.1
• Coordinates: 1091226..1091402 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FORC81_RS05345
• Protein ID: WP_001270286.1
• Coordinates: 1090764..1091180 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC81_RS05325	1085916..1086857	-	942	WP_001251304.1	ABC transporter permease	-
FORC81_RS05330	1086858..1087871	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
FORC81_RS05335	1087889..1089034	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FORC81_RS05340	1089279..1090685	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
FORC81_RS05345	1090764..1091180	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FORC81_RS05350	1091226..1091402	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FORC81_RS05355	1091624..1091854	+	231	WP_000494244.1	YncJ family protein	-
FORC81_RS05360	1091946..1093907	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FORC81_RS05365	1093980..1094516	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FORC81_RS05370	1094569..1095783	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.007	flank	IS/Tn	-	-	1095823..1096971	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T104651 WP_000813794.1 NZ_CP029057:c1091402-1091226 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T104651 NZ_CP029057:c1091402-1091226 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT104651 WP_001270286.1 NZ_CP029057:c1091180-1090764 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT104651 NZ_CP029057:c1091180-1090764 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB