Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2508204..2508842 Replicon chromosome
Accession NZ_CP046003
Organism Escherichia coli strain 1916D6

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GJD94_RS12895 Protein ID WP_000813794.1
Coordinates 2508666..2508842 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GJD94_RS12890 Protein ID WP_001270286.1
Coordinates 2508204..2508620 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD94_RS12870 2503356..2504297 - 942 WP_001251304.1 ABC transporter permease -
GJD94_RS12875 2504298..2505311 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GJD94_RS12880 2505329..2506474 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GJD94_RS12885 2506719..2508125 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GJD94_RS12890 2508204..2508620 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GJD94_RS12895 2508666..2508842 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GJD94_RS12900 2509064..2509294 + 231 WP_000494244.1 YncJ family protein -
GJD94_RS12905 2509386..2511347 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GJD94_RS12910 2511420..2511956 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GJD94_RS12915 2512009..2513223 + 1215 WP_074400818.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2513263..2514411 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T143311 WP_000813794.1 NZ_CP046003:c2508842-2508666 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T143311 NZ_CP061029:2273318-2273413 [Staphylococcus epidermidis]
ATGGCAGATATTCTTGTTAATATCATGACCACGGCAGCAAGTGGTTGTATCGTCGCGTTGTTTTCATATTGGCTTCGAAA
ACGTGATGATAAATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT143311 WP_001270286.1 NZ_CP046003:c2508620-2508204 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT143311 NZ_CP061029:c2273474-2273438 [Staphylococcus epidermidis]
ATGCACCAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References