Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 300315..300953 Replicon chromosome
Accession NZ_CP021732
Organism Escherichia coli strain AR_0114

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag AM361_RS01510 Protein ID WP_001447010.1
Coordinates 300777..300953 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AM361_RS01505 Protein ID WP_001270286.1
Coordinates 300315..300731 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM361_RS01485 295467..296408 - 942 WP_001251304.1 ABC transporter permease -
AM361_RS01490 296409..297422 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
AM361_RS01495 297440..298585 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AM361_RS01500 298830..300236 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AM361_RS01505 300315..300731 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AM361_RS01510 300777..300953 - 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AM361_RS01515 301175..301405 + 231 WP_000494244.1 YncJ family protein -
AM361_RS01520 301497..303458 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AM361_RS01525 303531..304067 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AM361_RS01530 304120..305334 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 305374..306522 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T77191 WP_001447010.1 NZ_CP021732:c300953-300777 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T77191 NZ_CP021732:c300953-300777 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT77191 WP_001270286.1 NZ_CP021732:c300731-300315 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT77191 NZ_CP021732:c300731-300315 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References