Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2129977..2130615 Replicon chromosome
Accession NZ_CP086338
Organism Escherichia coli strain EJN001

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag C5N09_RS10315 Protein ID WP_001447010.1
Coordinates 2129977..2130153 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C5N09_RS10320 Protein ID WP_001270286.1
Coordinates 2130199..2130615 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N09_RS10295 (2125596) 2125596..2126771 - 1176 WP_001236265.1 BenE family transporter YdcO -
C5N09_RS10300 (2126863) 2126863..2127399 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C5N09_RS10305 (2127472) 2127472..2129433 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C5N09_RS10310 (2129525) 2129525..2129755 - 231 WP_000494244.1 YncJ family protein -
C5N09_RS10315 (2129977) 2129977..2130153 + 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C5N09_RS10320 (2130199) 2130199..2130615 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C5N09_RS10325 (2130694) 2130694..2132100 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
C5N09_RS10330 (2132345) 2132345..2133490 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
C5N09_RS10335 (2133508) 2133508..2134521 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C5N09_RS10340 (2134522) 2134522..2135463 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2124408..2125556 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T223035 WP_001447010.1 NZ_CP086338:2129977-2130153 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T223035 NZ_CP118279:31732-32046 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT223035 WP_001270286.1 NZ_CP086338:2130199-2130615 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT223035 NZ_CP118279:32046-32462 [Enterobacter hormaechei]
ATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGTGCG
CCAGAATTAGCCGTTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCACCTG
CGTTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References