Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1426763..1427401 Replicon chromosome
Accession NZ_CP077969
Organism Escherichia coli strain JM83

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KTJ86_RS06970 Protein ID WP_000813794.1
Coordinates 1426763..1426939 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KTJ86_RS06975 Protein ID WP_001270286.1
Coordinates 1426985..1427401 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTJ86_RS06950 (1422382) 1422382..1423596 - 1215 WP_001326689.1 BenE family transporter YdcO -
KTJ86_RS06955 (1423649) 1423649..1424185 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KTJ86_RS06960 (1424258) 1424258..1426219 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KTJ86_RS06965 (1426311) 1426311..1426541 - 231 WP_000494244.1 YncJ family protein -
KTJ86_RS06970 (1426763) 1426763..1426939 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KTJ86_RS06975 (1426985) 1426985..1427401 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KTJ86_RS06980 (1427480) 1427480..1428886 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KTJ86_RS06985 (1429131) 1429131..1430276 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
KTJ86_RS06990 (1430294) 1430294..1431307 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
KTJ86_RS06995 (1431308) 1431308..1432249 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1421194..1422342 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T208755 WP_000813794.1 NZ_CP077969:1426763-1426939 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T208755 NZ_CP103720:11245-11394 [Escherichia coli]
CTGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT208755 WP_001270286.1 NZ_CP077969:1426985-1427401 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT208755 NZ_CP103720:c11200-11141 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References