Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 538539..539177 Replicon chromosome
Accession NZ_CP079780
Organism Escherichia coli strain BL12

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KYX59_RS04660 Protein ID WP_000813794.1
Coordinates 538539..538715 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KYX59_RS04665 Protein ID WP_001270286.1
Coordinates 538761..539177 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX59_RS04640 (534158) 534158..535333 - 1176 WP_032253412.1 BenE family transporter YdcO -
KYX59_RS04645 (535425) 535425..535961 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KYX59_RS04650 (536034) 536034..537995 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KYX59_RS04655 (538087) 538087..538317 - 231 WP_000494244.1 YncJ family protein -
KYX59_RS04660 (538539) 538539..538715 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KYX59_RS04665 (538761) 538761..539177 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KYX59_RS04670 (539256) 539256..540662 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KYX59_RS04675 (540907) 540907..542052 + 1146 WP_000047418.1 ABC transporter substrate-binding protein -
KYX59_RS04680 (542070) 542070..543083 + 1014 WP_000220393.1 ABC transporter ATP-binding protein -
KYX59_RS04685 (543084) 543084..544025 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 532970..534118 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T210059 WP_000813794.1 NZ_CP079780:538539-538715 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T210059 NZ_CP104384:182016-182123 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT210059 WP_001270286.1 NZ_CP079780:538761-539177 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT210059 NZ_CP104384:c181968-181902 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References