Detailed information of TA system TA043860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4466065..4466703
• Replicon: chromosome
• Accession: NZ_AP027411
• Organism: Escherichia coli strain EC521 isolate EC521

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QUE69_RS22330
• Protein ID: WP_000813794.1
• Coordinates: 4466527..4466703 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUE69_RS22325
• Protein ID: WP_001270286.1
• Coordinates: 4466065..4466481 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE69_RS22305 (4461217)	4461217..4462158	-	942	WP_001251304.1	ABC transporter permease	-
QUE69_RS22310 (4462159)	4462159..4463172	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QUE69_RS22315 (4463190)	4463190..4464335	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
QUE69_RS22320 (4464580)	4464580..4465986	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
QUE69_RS22325 (4466065)	4466065..4466481	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUE69_RS22330 (4466527)	4466527..4466703	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUE69_RS22335 (4466925)	4466925..4467155	+	231	WP_000494244.1	YncJ family protein	-
QUE69_RS22340 (4467247)	4467247..4469208	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUE69_RS22345 (4469281)	4469281..4469817	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QUE69_RS22350 (4469870)	4469870..4471084	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.007	flank	IS/Tn	-	-	4471124..4472272	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43860 WP_000813794.1 NZ_AP027411:c4466703-4466527 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43860 NZ_AP027411:c4466703-4466527 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43860 WP_001270286.1 NZ_AP027411:c4466481-4466065 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43860 NZ_AP027411:c4466481-4466065 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB