Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4466065..4466703 Replicon chromosome
Accession NZ_AP027411
Organism Escherichia coli strain EC521 isolate EC521

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QUE69_RS22330 Protein ID WP_000813794.1
Coordinates 4466527..4466703 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QUE69_RS22325 Protein ID WP_001270286.1
Coordinates 4466065..4466481 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE69_RS22305 (4461217) 4461217..4462158 - 942 WP_001251304.1 ABC transporter permease -
QUE69_RS22310 (4462159) 4462159..4463172 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
QUE69_RS22315 (4463190) 4463190..4464335 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
QUE69_RS22320 (4464580) 4464580..4465986 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
QUE69_RS22325 (4466065) 4466065..4466481 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QUE69_RS22330 (4466527) 4466527..4466703 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QUE69_RS22335 (4466925) 4466925..4467155 + 231 WP_000494244.1 YncJ family protein -
QUE69_RS22340 (4467247) 4467247..4469208 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QUE69_RS22345 (4469281) 4469281..4469817 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QUE69_RS22350 (4469870) 4469870..4471084 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4471124..4472272 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T43860 WP_000813794.1 NZ_AP027411:c4466703-4466527 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T43860 NZ_AP027411:c4466703-4466527 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT43860 WP_001270286.1 NZ_AP027411:c4466481-4466065 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT43860 NZ_AP027411:c4466481-4466065 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References