Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 325892..326530 Replicon chromosome
Accession NZ_CP043217
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FYA02_RS01740 Protein ID WP_000813794.1
Coordinates 325892..326068 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FYA02_RS01745 Protein ID WP_001270286.1
Coordinates 326114..326530 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS01720 321511..322725 - 1215 WP_001326689.1 BenE family transporter YdcO -
FYA02_RS01725 322778..323314 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FYA02_RS01730 323387..325348 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FYA02_RS01735 325440..325670 - 231 WP_000494244.1 YncJ family protein -
FYA02_RS01740 325892..326068 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FYA02_RS01745 326114..326530 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FYA02_RS01750 326609..328018 + 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
FYA02_RS01755 328260..329405 + 1146 WP_000047429.1 ABC transporter substrate-binding protein -
FYA02_RS01760 329423..330436 + 1014 WP_000220393.1 ABC transporter ATP-binding protein -
FYA02_RS01765 330437..331378 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 320323..321471 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T137936 WP_000813794.1 NZ_CP043217:325892-326068 [Escherichia coli O80:H26]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T137936 NZ_CP043217:325892-326068 [Escherichia coli O80:H26]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT137936 WP_001270286.1 NZ_CP043217:326114-326530 [Escherichia coli O80:H26]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT137936 NZ_CP043217:326114-326530 [Escherichia coli O80:H26]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References