Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3350184..3350822 Replicon chromosome
Accession NZ_CP084529
Organism Escherichia coli strain Origami2-DE3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LFW28_RS15935 Protein ID WP_000813794.1
Coordinates 3350184..3350360 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LFW28_RS15940 Protein ID WP_001270286.1
Coordinates 3350406..3350822 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFW28_RS15915 (3345803) 3345803..3347017 - 1215 WP_001326689.1 BenE family transporter YdcO -
LFW28_RS15920 (3347070) 3347070..3347606 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LFW28_RS15925 (3347679) 3347679..3349640 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LFW28_RS15930 (3349732) 3349732..3349962 - 231 WP_000494244.1 YncJ family protein -
LFW28_RS15935 (3350184) 3350184..3350360 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LFW28_RS15940 (3350406) 3350406..3350822 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LFW28_RS15945 (3350901) 3350901..3352307 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LFW28_RS15950 (3352552) 3352552..3353697 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LFW28_RS15955 (3353715) 3353715..3354728 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
LFW28_RS15960 (3354729) 3354729..3355670 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3344615..3345763 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T218807 WP_000813794.1 NZ_CP084529:3350184-3350360 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T218807 NZ_CP116103:c2607715-2607613 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT218807 WP_001270286.1 NZ_CP084529:3350406-3350822 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT218807 NZ_CP116103:2607575-2607843 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References