Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2458759..2459397 Replicon chromosome
Accession NZ_AP021893
Organism Escherichia coli strain 2018-06-4CC

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HRR20_RS11750 Protein ID WP_000813794.1
Coordinates 2459221..2459397 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HRR20_RS11745 Protein ID WP_001270286.1
Coordinates 2458759..2459175 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR20_RS11725 2453911..2454852 - 942 WP_001251319.1 ABC transporter permease -
HRR20_RS11730 2454853..2455866 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
HRR20_RS11735 2455884..2457029 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
HRR20_RS11740 2457271..2458680 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
HRR20_RS11745 2458759..2459175 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HRR20_RS11750 2459221..2459397 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HRR20_RS11755 2459619..2459849 + 231 WP_000494244.1 YncJ family protein -
HRR20_RS11760 2459941..2461902 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HRR20_RS11765 2461975..2462511 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HRR20_RS11770 2462564..2463778 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2463818..2464966 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T33604 WP_000813794.1 NZ_AP021893:c2459397-2459221 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T33604 NZ_AP021893:c2459397-2459221 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT33604 WP_001270286.1 NZ_AP021893:c2459175-2458759 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT33604 NZ_AP021893:c2459175-2458759 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References