Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1180116..1180754 Replicon chromosome
Accession NZ_CP063499
Organism Escherichia coli strain EH10-18-47

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IQS20_RS05610 Protein ID WP_000813794.1
Coordinates 1180578..1180754 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IQS20_RS05605 Protein ID WP_001270286.1
Coordinates 1180116..1180532 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS20_RS05585 1175268..1176209 - 942 WP_001251319.1 ABC transporter permease -
IQS20_RS05590 1176210..1177223 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
IQS20_RS05595 1177241..1178386 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
IQS20_RS05600 1178628..1180037 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
IQS20_RS05605 1180116..1180532 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IQS20_RS05610 1180578..1180754 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IQS20_RS05615 1180976..1181206 + 231 WP_000494244.1 YncJ family protein -
IQS20_RS05620 1181298..1183259 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IQS20_RS05625 1183332..1183868 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IQS20_RS05630 1183921..1185135 + 1215 WP_096282814.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1185175..1186323 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T178522 WP_000813794.1 NZ_CP063499:c1180754-1180578 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T178522 NZ_CP084096:381081-381608 [Escherichia coli O78]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT178522 WP_001270286.1 NZ_CP063499:c1180532-1180116 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT178522 NZ_CP084096:380808-381074 [Escherichia coli O78]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATTCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References