Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2155873..2156511 Replicon chromosome
Accession NZ_CP063473
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IQS24_RS10580 Protein ID WP_000813794.1
Coordinates 2155873..2156049 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IQS24_RS10585 Protein ID WP_001270286.1
Coordinates 2156095..2156511 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS10560 2151492..2152706 - 1215 WP_001326689.1 BenE family transporter YdcO -
IQS24_RS10565 2152759..2153295 + 537 WP_112915658.1 DNA-binding transcriptional regulator SutR -
IQS24_RS10570 2153368..2155329 + 1962 WP_147699513.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IQS24_RS10575 2155421..2155651 - 231 WP_000494244.1 YncJ family protein -
IQS24_RS10580 2155873..2156049 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IQS24_RS10585 2156095..2156511 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IQS24_RS10590 2156590..2157996 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
IQS24_RS10595 2158241..2159386 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
IQS24_RS10600 2159404..2160417 + 1014 WP_097729618.1 ABC transporter ATP-binding protein -
IQS24_RS10605 2160418..2161359 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2150304..2151452 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T178336 WP_000813794.1 NZ_CP063473:2155873-2156049 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T178336 NZ_CP083950:2507991-2508287 [Mesorhizobium sp. B4-1-4]
GTGATCGTCAGATTTCGAGACGAATGGCTGCGGGCGTTCTTCGTGGAAGATATCCCTTCCCGCAACATTCCGCCCGATCT
GGAAAGCCGGCTATTCCGTAAACTCCAGATGATCGATGACGCGATGACTGATCAGGATTTGGGTGTGCCGCCTAGCAATC
ATTTCGAGAAGTTGCGTGGCAATCTCGATGGCTTTCATTCAATCCGCGTCAACAAACAGTGGCGGCTGATCTTCCGGTGG
GATGGCGGTCGCGGTGAGGCGGCGGATATCTATCTCGACGACCATAGCTATAAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT178336 WP_001270286.1 NZ_CP063473:2156095-2156511 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT178336 NZ_CP083950:2508296-2508631 [Mesorhizobium sp. B4-1-4]
ATGTTGATGACCACGCGCAAGCCAGCAACGGTTGGTGAAATCCTCACGGAAGAATTCATGCAACCGCTCGGCCTGACACA
GGCGGCTTTGGCCGAGGCGATGGGTGTTCAGCGCAAGCATGTCAACGAATTGTGCAATGACCGCCGCAACGTGACCGCAG
CGACCGCGCTCATTCTGGCTCGGGTGTTTGGCAACAGCCCGGACTTCTGGCTGAACGTGCAGCGCCGTAGCGATCTCTGG
CAAGTCATGAATTCGCCTGTCGAGCGAGCGCGCGTCGATCGCGCCAAGCCTTTGGCGACAGCAGCGCAGATGACACGACA
ATCGGGCGCGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References