Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4375999..4376637 Replicon chromosome
Accession NZ_CP013952
Organism Escherichia coli strain HST04

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AWH44_RS22600 Protein ID WP_000813794.1
Coordinates 4376461..4376637 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AWH44_RS22595 Protein ID WP_001270286.1
Coordinates 4375999..4376415 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWH44_RS22575 4371151..4372092 - 942 WP_001251304.1 ABC transporter permease -
AWH44_RS22580 4372093..4373106 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
AWH44_RS22585 4373124..4374269 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AWH44_RS22590 4374514..4375920 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AWH44_RS22595 4375999..4376415 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AWH44_RS22600 4376461..4376637 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AWH44_RS22605 4376859..4377089 + 231 WP_000494244.1 YncJ family protein -
AWH44_RS22610 4377181..4379142 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AWH44_RS22615 4379215..4379751 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AWH44_RS22620 4379804..4381018 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4381058..4382206 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T59673 WP_000813794.1 NZ_CP013952:c4376637-4376461 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T59673 NZ_CP013952:c4376637-4376461 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT59673 WP_001270286.1 NZ_CP013952:c4376415-4375999 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT59673 NZ_CP013952:c4376415-4375999 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References