Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2436818..2437456 Replicon chromosome
Accession NZ_CP041452
Organism Escherichia coli strain YPE3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FNP78_RS12500 Protein ID WP_000813794.1
Coordinates 2437280..2437456 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FNP78_RS12495 Protein ID WP_001270286.1
Coordinates 2436818..2437234 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNP78_RS12475 2431970..2432911 - 942 WP_001251304.1 ABC transporter permease -
FNP78_RS12480 2432912..2433925 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FNP78_RS12485 2433943..2435088 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FNP78_RS12490 2435333..2436739 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FNP78_RS12495 2436818..2437234 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FNP78_RS12500 2437280..2437456 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FNP78_RS12505 2437678..2437908 + 231 WP_000494244.1 YncJ family protein -
FNP78_RS12510 2438000..2439961 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FNP78_RS12515 2440034..2440570 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FNP78_RS12520 2440623..2441837 + 1215 WP_074400818.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2441877..2443025 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T128683 WP_000813794.1 NZ_CP041452:c2437456-2437280 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T128683 NZ_CP041452:c2437456-2437280 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT128683 WP_001270286.1 NZ_CP041452:c2437234-2436818 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT128683 NZ_CP041452:c2437234-2436818 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References