Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2475362..2476000 Replicon chromosome
Accession NZ_CP075469
Organism Escherichia coli strain NT1W22

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IGI32_RS12070 Protein ID WP_000813794.1
Coordinates 2475824..2476000 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IGI32_RS12065 Protein ID WP_001270286.1
Coordinates 2475362..2475778 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI32_RS12045 (2470514) 2470514..2471455 - 942 WP_001251304.1 ABC transporter permease -
IGI32_RS12050 (2471456) 2471456..2472469 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
IGI32_RS12055 (2472487) 2472487..2473632 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
IGI32_RS12060 (2473877) 2473877..2475283 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
IGI32_RS12065 (2475362) 2475362..2475778 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IGI32_RS12070 (2475824) 2475824..2476000 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IGI32_RS12075 (2476222) 2476222..2476452 + 231 WP_000494244.1 YncJ family protein -
IGI32_RS12080 (2476544) 2476544..2478505 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IGI32_RS12085 (2478578) 2478578..2479114 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IGI32_RS12090 (2479206) 2479206..2480381 + 1176 WP_064670497.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2480421..2481569 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T203660 WP_000813794.1 NZ_CP075469:c2476000-2475824 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T203660 NZ_CP100232:c123326-122925 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT203660 WP_001270286.1 NZ_CP075469:c2475778-2475362 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT203660 NZ_CP100232:c123562-123326 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References