Detailed information of TA system TA044229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2458249..2458887
• Replicon: chromosome
• Accession: NZ_AP027953
• Organism: Escherichia coli strain VE-F5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: QQJ99_RS12055
• Protein ID: WP_001447010.1
• Coordinates: 2458711..2458887 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QQJ99_RS12050
• Protein ID: WP_001270286.1
• Coordinates: 2458249..2458665 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQJ99_RS12030 (2453401)	2453401..2454342	-	942	WP_001251304.1	ABC transporter permease	-
QQJ99_RS12035 (2454343)	2454343..2455356	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QQJ99_RS12040 (2455374)	2455374..2456519	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
QQJ99_RS12045 (2456764)	2456764..2458170	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
QQJ99_RS12050 (2458249)	2458249..2458665	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QQJ99_RS12055 (2458711)	2458711..2458887	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QQJ99_RS12060 (2459109)	2459109..2459339	+	231	WP_000494244.1	YncJ family protein	-
QQJ99_RS12065 (2459431)	2459431..2461392	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QQJ99_RS12070 (2461465)	2461465..2462001	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QQJ99_RS12075 (2462054)	2462054..2463268	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.008	flank	IS/Tn	-	-	2463308..2464456	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T44229 WP_001447010.1 NZ_AP027953:c2458887-2458711 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T44229 NZ_AP027953:c2458887-2458711 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT44229 WP_001270286.1 NZ_AP027953:c2458665-2458249 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT44229 NZ_AP027953:c2458665-2458249 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8