Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2458249..2458887 Replicon chromosome
Accession NZ_AP027953
Organism Escherichia coli strain VE-F5

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag QQJ99_RS12055 Protein ID WP_001447010.1
Coordinates 2458711..2458887 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QQJ99_RS12050 Protein ID WP_001270286.1
Coordinates 2458249..2458665 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQJ99_RS12030 (2453401) 2453401..2454342 - 942 WP_001251304.1 ABC transporter permease -
QQJ99_RS12035 (2454343) 2454343..2455356 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
QQJ99_RS12040 (2455374) 2455374..2456519 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
QQJ99_RS12045 (2456764) 2456764..2458170 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
QQJ99_RS12050 (2458249) 2458249..2458665 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QQJ99_RS12055 (2458711) 2458711..2458887 - 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QQJ99_RS12060 (2459109) 2459109..2459339 + 231 WP_000494244.1 YncJ family protein -
QQJ99_RS12065 (2459431) 2459431..2461392 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QQJ99_RS12070 (2461465) 2461465..2462001 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QQJ99_RS12075 (2462054) 2462054..2463268 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2463308..2464456 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T44229 WP_001447010.1 NZ_AP027953:c2458887-2458711 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T44229 NZ_AP027953:c2458887-2458711 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT44229 WP_001270286.1 NZ_AP027953:c2458665-2458249 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT44229 NZ_AP027953:c2458665-2458249 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References