Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1257609..1258247 Replicon chromosome
Accession NZ_CP063369
Organism Escherichia coli strain 1409

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FOI11_RS05955 Protein ID WP_000813794.1
Coordinates 1258071..1258247 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FOI11_RS05950 Protein ID WP_001270286.1
Coordinates 1257609..1258025 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOI11_RS05930 1252761..1253702 - 942 WP_001251304.1 ABC transporter permease -
FOI11_RS05935 1253703..1254716 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FOI11_RS05940 1254734..1255879 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FOI11_RS05945 1256124..1257530 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FOI11_RS05950 1257609..1258025 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FOI11_RS05955 1258071..1258247 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FOI11_RS05960 1258469..1258699 + 231 WP_000494244.1 YncJ family protein -
FOI11_RS05965 1258791..1260752 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FOI11_RS05970 1260825..1261361 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FOI11_RS05975 1261414..1262628 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1262668..1263816 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T178037 WP_000813794.1 NZ_CP063369:c1258247-1258071 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T178037 NZ_CP083821:543088-543372 [Enterobacter cloacae subsp. cloacae]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAAGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAG
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCTGCATAATCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGATAGTTGTCATCGCTATTGGGAAA
AGAGAGAAATTAACCGTGTATCACGATGCTAACAAACGGCTATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT178037 WP_001270286.1 NZ_CP063369:c1258025-1257609 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT178037 NZ_CP083821:542856-543098 [Enterobacter cloacae subsp. cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTGTTTCAGTACGTTGCGGAGACGGGGCGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References