Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 595363..596001 Replicon chromosome
Accession NZ_CP042336
Organism Escherichia coli strain GZ04-0086

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag E3B71_RS04890 Protein ID WP_001447010.1
Coordinates 595363..595539 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag E3B71_RS04895 Protein ID WP_001270286.1
Coordinates 595585..596001 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3B71_RS04870 590982..592196 - 1215 WP_001326689.1 BenE family transporter YdcO -
E3B71_RS04875 592249..592785 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
E3B71_RS04880 592858..594819 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
E3B71_RS04885 594911..595141 - 231 WP_000494244.1 YncJ family protein -
E3B71_RS04890 595363..595539 + 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
E3B71_RS04895 595585..596001 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
E3B71_RS04900 596080..597486 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
E3B71_RS04905 597731..598876 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
E3B71_RS04910 598894..599907 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
E3B71_RS04915 599908..600849 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 589794..590942 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T135405 WP_001447010.1 NZ_CP042336:595363-595539 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T135405 NZ_CP042336:595363-595539 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT135405 WP_001270286.1 NZ_CP042336:595585-596001 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT135405 NZ_CP042336:595585-596001 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References