Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1748018..1748656 Replicon chromosome
Accession NC_017641
Organism Escherichia coli UMNK88

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag UMNK88_RS09065 Protein ID WP_000813794.1
Coordinates 1748018..1748194 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag UMNK88_RS09070 Protein ID WP_001270286.1
Coordinates 1748240..1748656 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UMNK88_RS09045 1743637..1744851 - 1215 WP_001326689.1 BenE family transporter YdcO -
UMNK88_RS09050 1744904..1745440 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
UMNK88_RS09055 1745513..1747474 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
UMNK88_RS09060 1747566..1747796 - 231 WP_000494244.1 YncJ family protein -
UMNK88_RS09065 1748018..1748194 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
UMNK88_RS09070 1748240..1748656 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
UMNK88_RS09075 1748735..1750144 + 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
UMNK88_RS09080 1750386..1751531 + 1146 WP_000047429.1 ABC transporter substrate-binding protein -
UMNK88_RS09085 1751549..1752562 + 1014 WP_000220393.1 ABC transporter ATP-binding protein -
UMNK88_RS09090 1752563..1753504 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1742449..1743597 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T27446 WP_000813794.1 NC_017641:1748018-1748194 [Escherichia coli UMNK88]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T27446 NC_017641:1748018-1748194 [Escherichia coli UMNK88]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT27446 WP_001270286.1 NC_017641:1748240-1748656 [Escherichia coli UMNK88]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT27446 NC_017641:1748240-1748656 [Escherichia coli UMNK88]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References