200193

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2476306..2476944 Replicon chromosome
Accession NZ_CP073768
Organism Escherichia coli strain SH19PTE6

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KEH54_RS12005 Protein ID WP_000813794.1
Coordinates 2476768..2476944 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KEH54_RS12000 Protein ID WP_001270286.1
Coordinates 2476306..2476722 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEH54_RS11980 (2471458) 2471458..2472399 - 942 WP_001251304.1 ABC transporter permease -
KEH54_RS11985 (2472400) 2472400..2473413 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
KEH54_RS11990 (2473431) 2473431..2474576 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
KEH54_RS11995 (2474821) 2474821..2476227 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KEH54_RS12000 (2476306) 2476306..2476722 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KEH54_RS12005 (2476768) 2476768..2476944 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KEH54_RS12010 (2477166) 2477166..2477396 + 231 WP_000494244.1 YncJ family protein -
KEH54_RS12015 (2477488) 2477488..2479449 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KEH54_RS12020 (2479522) 2479522..2480058 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KEH54_RS12025 (2480111) 2480111..2481325 + 1215 WP_074400818.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2481365..2482513 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T200193 WP_000813794.1 NZ_CP073768:c2476944-2476768 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T200193 NZ_CP097721:1837779-1838030 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT200193 WP_001270286.1 NZ_CP073768:c2476722-2476306 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT200193 NZ_CP097721:1838027-1838281 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References