Detailed information of TA system TA198065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2488596..2489234
• Replicon: chromosome
• Accession: NZ_CP072564
• Organism: Escherichia coli strain WG5::PA2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J8F87_RS12025
• Protein ID: WP_000813794.1
• Coordinates: 2489058..2489234 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J8F87_RS12020
• Protein ID: WP_001270286.1
• Coordinates: 2488596..2489012 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8F87_RS12000 (2483748)	2483748..2484689	-	942	WP_001251304.1	ABC transporter permease	-
J8F87_RS12005 (2484690)	2484690..2485703	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
J8F87_RS12010 (2485721)	2485721..2486866	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
J8F87_RS12015 (2487111)	2487111..2488517	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
J8F87_RS12020 (2488596)	2488596..2489012	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J8F87_RS12025 (2489058)	2489058..2489234	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J8F87_RS12030 (2489456)	2489456..2489686	+	231	WP_000494244.1	YncJ family protein	-
J8F87_RS12035 (2489778)	2489778..2491739	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J8F87_RS12040 (2491812)	2491812..2492348	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
J8F87_RS12045 (2492401)	2492401..2493615	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0	flank	IS/Tn	-	-	2493655..2494803	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T198065 WP_000813794.1 NZ_CP072564:c2489234-2489058 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T198065 NZ_CP096840:c2822737-2822318 [Mycobacterium tuberculosis variant bovis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT198065 WP_001270286.1 NZ_CP072564:c2489012-2488596 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT198065 NZ_CP096840:c2822958-2822734 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• PDB: 6HPB