Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2388849..2389487 Replicon chromosome
Accession NZ_AP026961
Organism Escherichia coli strain EC20-72C-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag OO975_RS11530 Protein ID WP_000813794.1
Coordinates 2389311..2389487 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag OO975_RS11525 Protein ID WP_001270286.1
Coordinates 2388849..2389265 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO975_RS11505 (2384001) 2384001..2384942 - 942 WP_001251319.1 ABC transporter permease -
OO975_RS11510 (2384943) 2384943..2385956 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
OO975_RS11515 (2385974) 2385974..2387119 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
OO975_RS11520 (2387364) 2387364..2388770 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
OO975_RS11525 (2388849) 2388849..2389265 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
OO975_RS11530 (2389311) 2389311..2389487 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
OO975_RS11535 (2389709) 2389709..2389939 + 231 WP_000494244.1 YncJ family protein -
OO975_RS11540 (2390031) 2390031..2391992 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
OO975_RS11545 (2392065) 2392065..2392601 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
OO975_RS11550 (2392654) 2392654..2393868 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2393908..2395056 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T42937 WP_000813794.1 NZ_AP026961:c2389487-2389311 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T42937 NZ_AP026961:c2389487-2389311 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT42937 WP_001270286.1 NZ_AP026961:c2389265-2388849 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT42937 NZ_AP026961:c2389265-2388849 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References