Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2579233..2579871 Replicon chromosome
Accession NZ_CP024127
Organism Escherichia coli strain 14EC001

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CR534_RS13795 Protein ID WP_000813794.1
Coordinates 2579695..2579871 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CR534_RS13790 Protein ID WP_001270286.1
Coordinates 2579233..2579649 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR534_RS13770 2574385..2575326 - 942 WP_001251319.1 ABC transporter permease -
CR534_RS13775 2575327..2576340 - 1014 WP_024238793.1 ABC transporter ATP-binding protein -
CR534_RS13780 2576358..2577503 - 1146 WP_101981576.1 ABC transporter substrate-binding protein -
CR534_RS13785 2577748..2579154 - 1407 WP_101981577.1 PLP-dependent aminotransferase family protein -
CR534_RS13790 2579233..2579649 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CR534_RS13795 2579695..2579871 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CR534_RS13800 2580093..2580323 + 231 WP_000494244.1 YncJ family protein -
CR534_RS13805 2580415..2582376 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CR534_RS13810 2582449..2582985 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CR534_RS13815 2583038..2584252 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2584292..2585440 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T86796 WP_000813794.1 NZ_CP024127:c2579871-2579695 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T86796 NZ_CP024127:c2579871-2579695 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT86796 WP_001270286.1 NZ_CP024127:c2579649-2579233 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT86796 NZ_CP024127:c2579649-2579233 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References