Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2425052..2425690 Replicon chromosome
Accession NZ_CP072566
Organism Escherichia coli strain WG5::TT12A

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J8F85_RS11595 Protein ID WP_000813794.1
Coordinates 2425514..2425690 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J8F85_RS11590 Protein ID WP_001270286.1
Coordinates 2425052..2425468 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F85_RS11570 (2420204) 2420204..2421145 - 942 WP_001251304.1 ABC transporter permease -
J8F85_RS11575 (2421146) 2421146..2422159 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
J8F85_RS11580 (2422177) 2422177..2423322 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
J8F85_RS11585 (2423567) 2423567..2424973 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J8F85_RS11590 (2425052) 2425052..2425468 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J8F85_RS11595 (2425514) 2425514..2425690 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J8F85_RS11600 (2425912) 2425912..2426142 + 231 WP_000494244.1 YncJ family protein -
J8F85_RS11605 (2426234) 2426234..2428195 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J8F85_RS11610 (2428268) 2428268..2428804 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
J8F85_RS11615 (2428896) 2428896..2430071 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2430111..2431259 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T198111 WP_000813794.1 NZ_CP072566:c2425690-2425514 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T198111 NZ_CP096841:c2183987-2183658 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT198111 WP_001270286.1 NZ_CP072566:c2425468-2425052 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT198111 NZ_CP096841:c2184361-2183984 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References