Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2544862..2545500 Replicon chromosome
Accession NZ_CP026929
Organism Escherichia coli strain CFS3246

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag C4962_RS12435 Protein ID WP_001447010.1
Coordinates 2545324..2545500 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C4962_RS12430 Protein ID WP_001270286.1
Coordinates 2544862..2545278 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4962_RS12410 2540014..2540955 - 942 WP_001251304.1 ABC transporter permease -
C4962_RS12415 2540956..2541969 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C4962_RS12420 2541987..2543132 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
C4962_RS12425 2543377..2544783 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
C4962_RS12430 2544862..2545278 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C4962_RS12435 2545324..2545500 - 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C4962_RS12440 2545722..2545952 + 231 WP_000494244.1 YncJ family protein -
C4962_RS12445 2546044..2548005 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C4962_RS12450 2548078..2548614 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C4962_RS12455 2548667..2549881 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2549921..2551069 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T96639 WP_001447010.1 NZ_CP026929:c2545500-2545324 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T96639 NZ_CP026929:c2545500-2545324 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT96639 WP_001270286.1 NZ_CP026929:c2545278-2544862 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT96639 NZ_CP026929:c2545278-2544862 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References