Detailed information of TA system TA074422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1445532..1446170
• Replicon: chromosome
• Accession: NZ_CP020368
• Organism: Escherichia coli strain BLR(DE3)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: B5762_RS07635
• Protein ID: WP_000813794.1
• Coordinates: 1445532..1445708 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: B5762_RS07640
• Protein ID: WP_001270286.1
• Coordinates: 1445754..1446170 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5762_RS07615	1441151..1442365	-	1215	WP_001326689.1	BenE family transporter YdcO	-
B5762_RS07620	1442418..1442954	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
B5762_RS07625	1443027..1444988	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
B5762_RS07630	1445080..1445310	-	231	WP_000494244.1	YncJ family protein	-
B5762_RS07635	1445532..1445708	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
B5762_RS07640	1445754..1446170	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
B5762_RS07645	1446249..1447655	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
B5762_RS07650	1447900..1449045	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
B5762_RS07655	1449063..1450076	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
B5762_RS07660	1450077..1451018	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.007	flank	IS/Tn	-	-	1439963..1441111	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T74422 WP_000813794.1 NZ_CP020368:1445532-1445708 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T74422 NZ_CP020368:1445532-1445708 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT74422 WP_001270286.1 NZ_CP020368:1445754-1446170 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT74422 NZ_CP020368:1445754-1446170 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB