Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2578776..2579414 Replicon chromosome
Accession NZ_CP024131
Organism Escherichia coli strain 14EC007

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CR535_RS13735 Protein ID WP_000813794.1
Coordinates 2579238..2579414 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CR535_RS13730 Protein ID WP_001270286.1
Coordinates 2578776..2579192 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR535_RS13710 2573928..2574869 - 942 WP_001251319.1 ABC transporter permease -
CR535_RS13715 2574870..2575883 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
CR535_RS13720 2575901..2577046 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
CR535_RS13725 2577288..2578697 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
CR535_RS13730 2578776..2579192 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CR535_RS13735 2579238..2579414 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CR535_RS13740 2579636..2579866 + 231 WP_000494244.1 YncJ family protein -
CR535_RS13745 2579958..2581919 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CR535_RS13750 2581992..2582528 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CR535_RS13755 2582581..2583795 + 1215 WP_101972910.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2583835..2584983 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T86822 WP_000813794.1 NZ_CP024131:c2579414-2579238 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T86822 NZ_CP024131:c2579414-2579238 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT86822 WP_001270286.1 NZ_CP024131:c2579192-2578776 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT86822 NZ_CP024131:c2579192-2578776 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References