Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2283983..2284621 Replicon chromosome
Accession NZ_CP029492
Organism Escherichia coli strain HS30-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag DKQ63_RS11715 Protein ID WP_000813794.1
Coordinates 2284445..2284621 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DKQ63_RS11710 Protein ID WP_001270286.1
Coordinates 2283983..2284399 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKQ63_RS11690 2279135..2280076 - 942 WP_001251319.1 ABC transporter permease -
DKQ63_RS11695 2280077..2281090 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
DKQ63_RS11700 2281108..2282253 - 1146 WP_110991468.1 ABC transporter substrate-binding protein -
DKQ63_RS11705 2282498..2283904 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
DKQ63_RS11710 2283983..2284399 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
DKQ63_RS11715 2284445..2284621 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
DKQ63_RS11720 2284843..2285073 + 231 WP_000494244.1 YncJ family protein -
DKQ63_RS11725 2285165..2287126 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
DKQ63_RS11730 2287199..2287735 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
DKQ63_RS11735 2287788..2289002 + 1215 WP_110991469.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2289042..2290190 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T105745 WP_000813794.1 NZ_CP029492:c2284621-2284445 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T105745 NZ_CP029492:c2284621-2284445 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT105745 WP_001270286.1 NZ_CP029492:c2284399-2283983 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT105745 NZ_CP029492:c2284399-2283983 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References