Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1527990..1528628 Replicon chromosome
Accession NZ_CP010371
Organism Escherichia coli strain 6409

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag RR31_RS07485 Protein ID WP_001447010.1
Coordinates 1527990..1528166 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RR31_RS07490 Protein ID WP_001270286.1
Coordinates 1528212..1528628 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR31_RS07465 1523609..1524823 - 1215 WP_001326689.1 BenE family transporter YdcO -
RR31_RS07470 1524876..1525412 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RR31_RS07475 1525485..1527446 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RR31_RS07480 1527538..1527768 - 231 WP_000494244.1 YncJ family protein -
RR31_RS07485 1527990..1528166 + 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RR31_RS07490 1528212..1528628 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RR31_RS07495 1528707..1530113 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
RR31_RS07500 1530358..1531503 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
RR31_RS07505 1531521..1532534 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
RR31_RS07510 1532535..1533476 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1522421..1523569 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T52234 WP_001447010.1 NZ_CP010371:1527990-1528166 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T52234 NZ_CP010371:1527990-1528166 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT52234 WP_001270286.1 NZ_CP010371:1528212-1528628 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT52234 NZ_CP010371:1528212-1528628 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References