Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 964987..965625 Replicon chromosome
Accession NZ_CP014641
Organism Escherichia coli strain BLK9

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AYL54_RS04985 Protein ID WP_000813794.1
Coordinates 964987..965163 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AYL54_RS04990 Protein ID WP_001270286.1
Coordinates 965209..965625 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYL54_RS04965 (960606) 960606..961781 - 1176 WP_001236265.1 BenE family transporter YdcO -
AYL54_RS04970 (961873) 961873..962409 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AYL54_RS04975 (962482) 962482..964443 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AYL54_RS04980 (964535) 964535..964765 - 231 WP_000494244.1 YncJ family protein -
AYL54_RS04985 (964987) 964987..965163 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AYL54_RS04990 (965209) 965209..965625 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AYL54_RS04995 (965704) 965704..967110 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AYL54_RS05000 (967355) 967355..968500 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AYL54_RS05005 (968518) 968518..969531 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
AYL54_RS05010 (969532) 969532..970473 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 959418..960566 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T61581 WP_000813794.1 NZ_CP014641:964987-965163 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T61581 NZ_CP014641:964987-965163 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT61581 WP_001270286.1 NZ_CP014641:965209-965625 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT61581 NZ_CP014641:965209-965625 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References