Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 909802..910440 Replicon chromosome
Accession NZ_CP058725
Organism Escherichia coli strain S171-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HZS87_RS04460 Protein ID WP_000813794.1
Coordinates 910264..910440 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HZS87_RS04455 Protein ID WP_001270286.1
Coordinates 909802..910218 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS87_RS04435 (904954) 904954..905895 - 942 WP_001251319.1 ABC transporter permease -
HZS87_RS04440 (905896) 905896..906909 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
HZS87_RS04445 (906927) 906927..908072 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
HZS87_RS04450 (908317) 908317..909723 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HZS87_RS04455 (909802) 909802..910218 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HZS87_RS04460 (910264) 910264..910440 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HZS87_RS04465 (910662) 910662..910892 + 231 WP_000494244.1 YncJ family protein -
HZS87_RS04470 (910984) 910984..912945 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HZS87_RS04475 (913018) 913018..913554 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HZS87_RS04480 (913646) 913646..914821 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 914861..916009 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T167280 WP_000813794.1 NZ_CP058725:c910440-910264 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T167280 NZ_CP058725:c910440-910264 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT167280 WP_001270286.1 NZ_CP058725:c910218-909802 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT167280 NZ_CP058725:c910218-909802 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References