Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2396948..2397586 Replicon chromosome
Accession NZ_CP073988
Organism Escherichia coli strain MB10

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KFU65_RS11735 Protein ID WP_000813794.1
Coordinates 2397410..2397586 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KFU65_RS11730 Protein ID WP_001270286.1
Coordinates 2396948..2397364 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU65_RS11710 (2392100) 2392100..2393041 - 942 WP_001251319.1 ABC transporter permease -
KFU65_RS11715 (2393042) 2393042..2394055 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
KFU65_RS11720 (2394073) 2394073..2395218 - 1146 WP_032265743.1 ABC transporter substrate-binding protein -
KFU65_RS11725 (2395460) 2395460..2396869 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
KFU65_RS11730 (2396948) 2396948..2397364 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KFU65_RS11735 (2397410) 2397410..2397586 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KFU65_RS11740 (2397808) 2397808..2398038 + 231 WP_000494244.1 YncJ family protein -
KFU65_RS11745 (2398130) 2398130..2400091 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KFU65_RS11750 (2400164) 2400164..2400700 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KFU65_RS11755 (2400792) 2400792..2401967 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2402007..2403155 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T200951 WP_000813794.1 NZ_CP073988:c2397586-2397410 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T200951 NZ_CP098201:c3916546-3916235 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT200951 WP_001270286.1 NZ_CP073988:c2397364-2396948 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT200951 NZ_CP098201:c3916238-3915819 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References