Detailed information of TA system TA050890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2114782..2115420
• Replicon: chromosome
• Accession: NZ_CP010152
• Organism: Escherichia coli strain D9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: RG42_RS11160
• Protein ID: WP_000813794.1
• Coordinates: 2114782..2114958 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: RG42_RS11165
• Protein ID: WP_001270286.1
• Coordinates: 2115004..2115420 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG42_RS11140	2110401..2111615	-	1215	WP_001326689.1	BenE family transporter YdcO	-
RG42_RS11145	2111668..2112204	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
RG42_RS11150	2112277..2114238	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
RG42_RS11155	2114330..2114560	-	231	WP_000494244.1	YncJ family protein	-
RG42_RS11160	2114782..2114958	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
RG42_RS11165	2115004..2115420	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
RG42_RS11170	2115499..2116905	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
RG42_RS11175	2117150..2118295	+	1146	WP_073503305.1	ABC transporter substrate-binding protein	-
RG42_RS11180	2118313..2119326	+	1014	WP_073503306.1	ABC transporter ATP-binding protein	-
RG42_RS11185	2119327..2120268	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.008	flank	IS/Tn	-	-	2109213..2110361	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T50890 WP_000813794.1 NZ_CP010152:2114782-2114958 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T50890 NZ_CP010152:2114782-2114958 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT50890 WP_001270286.1 NZ_CP010152:2115004-2115420 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT50890 NZ_CP010152:2115004-2115420 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB