Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2114782..2115420 Replicon chromosome
Accession NZ_CP010152
Organism Escherichia coli strain D9

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG42_RS11160 Protein ID WP_000813794.1
Coordinates 2114782..2114958 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG42_RS11165 Protein ID WP_001270286.1
Coordinates 2115004..2115420 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG42_RS11140 2110401..2111615 - 1215 WP_001326689.1 BenE family transporter YdcO -
RG42_RS11145 2111668..2112204 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RG42_RS11150 2112277..2114238 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG42_RS11155 2114330..2114560 - 231 WP_000494244.1 YncJ family protein -
RG42_RS11160 2114782..2114958 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG42_RS11165 2115004..2115420 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG42_RS11170 2115499..2116905 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
RG42_RS11175 2117150..2118295 + 1146 WP_073503305.1 ABC transporter substrate-binding protein -
RG42_RS11180 2118313..2119326 + 1014 WP_073503306.1 ABC transporter ATP-binding protein -
RG42_RS11185 2119327..2120268 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2109213..2110361 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T50890 WP_000813794.1 NZ_CP010152:2114782-2114958 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T50890 NZ_CP010152:2114782-2114958 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT50890 WP_001270286.1 NZ_CP010152:2115004-2115420 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT50890 NZ_CP010152:2115004-2115420 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References