Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2427311..2427949 Replicon chromosome
Accession NZ_LT906474
Organism Escherichia coli strain NCTC122

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CKV98_RS12310 Protein ID WP_000813794.1
Coordinates 2427773..2427949 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CKV98_RS12305 Protein ID WP_001270286.1
Coordinates 2427311..2427727 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKV98_RS12285 2422463..2423404 - 942 WP_001251304.1 ABC transporter permease -
CKV98_RS12290 2423405..2424418 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
CKV98_RS12295 2424436..2425581 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
CKV98_RS12300 2425826..2427232 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
CKV98_RS12305 2427311..2427727 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CKV98_RS12310 2427773..2427949 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CKV98_RS12315 2428171..2428401 + 231 WP_000494244.1 YncJ family protein -
CKV98_RS12320 2428493..2430454 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CKV98_RS12325 2430527..2431063 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CKV98_RS12330 2431116..2432330 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2432370..2433518 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T293755 WP_000813794.1 NZ_LT906474:c2427949-2427773 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT293755 WP_001270286.1 NZ_LT906474:c2427727-2427311 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References