Detailed information of TA system TA200608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2472048..2472686
• Replicon: chromosome
• Accession: NZ_CP073929
• Organism: Escherichia coli strain MB64

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU91_RS11780
• Protein ID: WP_000813794.1
• Coordinates: 2472510..2472686 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU91_RS11775
• Protein ID: WP_001270286.1
• Coordinates: 2472048..2472464 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU91_RS11755 (2467200)	2467200..2468141	-	942	WP_001251319.1	ABC transporter permease	-
KFU91_RS11760 (2468142)	2468142..2469155	-	1014	WP_104769175.1	ABC transporter ATP-binding protein	-
KFU91_RS11765 (2469173)	2469173..2470318	-	1146	WP_000047429.1	ABC transporter substrate-binding protein	-
KFU91_RS11770 (2470560)	2470560..2471969	-	1410	WP_000760625.1	PLP-dependent aminotransferase family protein	-
KFU91_RS11775 (2472048)	2472048..2472464	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU91_RS11780 (2472510)	2472510..2472686	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU91_RS11785 (2472908)	2472908..2473138	+	231	WP_000494244.1	YncJ family protein	-
KFU91_RS11790 (2473230)	2473230..2475191	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU91_RS11795 (2475264)	2475264..2475800	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KFU91_RS11800 (2475853)	2475853..2477067	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.007	flank	IS/Tn	-	-	2477107..2478255	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T200608 WP_000813794.1 NZ_CP073929:c2472686-2472510 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200608 NZ_CP098040:c1930162-1929845 [Providencia rettgeri]
TTGACTCAGAAGCCAAGTAATAAGCGAGCTAAGCAACCAAGGCAGGTTGCTTATACACCAACGTTTAAGAAGTCATGGGA
ACGATATAACCGAGCGGGCCGTAGGGATATGAATGCGGCTGTTCTCGTTATGGAAATACTGTTTTCTCAAAAAATAATAC
CAAAAGAATATCTTGACCATGAATTAGAAGGTCATGAATGGCAAGGAGCTAGGGAGCTTCATATTGGTGGTGATTTCTTA
CTGGTTTATCGCTTGTCAGATAAAGGTAATTTAATCACTTTCGTTGATATTGGTTCGCACAGTGAATTATTTGGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT200608 WP_001270286.1 NZ_CP073929:c2472464-2472048 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200608 NZ_CP098040:c1930430-1930143 [Providencia rettgeri]
ATGTCAACACTATTGAAAACAACAGATGTTCGTAGTCGAATTGATGAACAATTAAAAGCGGAAGCCACGAGTGTATTACA
AGATTGTGGTTTGACTATTAGCGCAGCTATTCGTTTGTTTTTAGAACAAGTAGTTCAAGAGCAAAGAGTTCCCTTTGAAA
TCAAACGTAAGCAACCTTCGCTGAAAACTACACTAGCGTTGAAAGAAGCTAAAGCTATTGAGCAGCACTACGATTCAATT
AACGAAATGATGGTGGAGTTAAGCAAAGTTGACTCAGAAGCCAAGTAA

Structures

Toxin

• PDB: 6HPB