Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 263837..264475 Replicon chromosome
Accession NZ_CP024859
Organism Escherichia coli strain AR_0014

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag AM341_RS01280 Protein ID WP_001447010.1
Coordinates 264299..264475 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AM341_RS01275 Protein ID WP_001270286.1
Coordinates 263837..264253 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM341_RS01255 258989..259930 - 942 WP_001251304.1 ABC transporter permease -
AM341_RS01260 259931..260944 - 1014 WP_100183743.1 ABC transporter ATP-binding protein -
AM341_RS01265 260962..262107 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AM341_RS01270 262352..263758 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AM341_RS01275 263837..264253 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AM341_RS01280 264299..264475 - 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AM341_RS01285 264697..264927 + 231 WP_000494244.1 YncJ family protein -
AM341_RS01290 265019..266980 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AM341_RS01295 267053..267589 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AM341_RS01300 267642..268856 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 268896..270044 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T88901 WP_001447010.1 NZ_CP024859:c264475-264299 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T88901 NZ_CP024859:c264475-264299 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT88901 WP_001270286.1 NZ_CP024859:c264253-263837 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT88901 NZ_CP024859:c264253-263837 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References