Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0019	PC1_3721 (Hfq)	T6SS01001	protein	QS regulatory pathway	Pectobacterium carotovorum subsp. carotovorum PC1	chromosome (CP001657)	4199890..4200189 (-)	251756660	hcp expression (↓)
REG0022	GPY04_RS03590 (TfoX)	T6SS01168	protein	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	777344..777952 (+)	487807468	Unknown (↓)
REG0023	GPY04_RS00870 (HapR)	T6SS01168	protein	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	164935..165546 (-)	446262243	Unknown (↓)
REG0025	Qrr1 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0026	Qrr2 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0027	Qrr3 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0028	Qrr4 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0029	GPY04_RS15835 (luxO)	T6SS01168	protein	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046845)	273631..274983 (-)	446367907	VC0070 (tsrA) (↑); GPY04_RS00870 (hapR) (↑)
REG0059	PA4704.1 (prrF1)	T6SS00003 ; T6SS00004	ncRNA	QS regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	5283960..5284110 (+)	4179187	T6SS transcripts (↓)
REG0060	PA4704.2 (prrF2)	T6SS00003 ; T6SS00004	ncRNA	QS regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	5284172..5284319 (+)	4179185	T6SS transcripts (↓)
REG0062	PA1244 (QslA)	T6SS00004	protein	QS regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	1348060..1348401 (-)	15596441	H2-T6SS (PA1656) promotor (↑)
REG0065	PA1430 (LasR)	T6SS00004	protein	Global regulator; QS regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	1558171..1558890 (+)	15596627	H2-T6SS promotor P (hsiA2) (↓)
REG0066	PA3476 (rhlI)	T6SS00004	protein	QS regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	3889139..3889744 (-)	15598672	PA3477 (RhlR) (↓)
REG0067	PA3477 (RhlR)	T6SS00004	protein	QS regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	3889925..3890650 (-)	15598673	T6SS transcript (↓)
REG0070	pa1663 (sfa2)	T6SS00004 ; T6SS00005	protein	Global regulator; QS regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	1813395..1814906 (+)	15596860	PA4462 (rpoN) (↑); T6SS transcript (↑); T6SS transcript (↓)
REG0093	ECA_RS00520 (expI)	T6SS00040	protein	QS regulatory pathway	Pectobacterium atrosepticum SCRI1043	chromosome (NC_004547)	125689..126339 (+)	499404281	T6SS transcripts (ECA4275[hcp1], ECA3428[hcp2] and ECA2866[hcp3]) (↓)
REG0111	VP2762 (AphA)	T6SS00043 ; T6SS00044	protein	QS regulatory pathway	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2929909..2930448 (+)	28899536	T6SS1 transcript (↑); T6SS2 (VPA1027-1024, VPA1043-1028,VPA1044-1046) transcripts (↑)
REG0112	VP2516 (opaR)	T6SS00043 ; T6SS00044	protein	QS regulatory pathway	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2653215..2653829 (+)	28899290	vp1407 (↑); VP1388 promotor (↑); VP1400 promotor (↑); VP1409 promotor (↑); T6SS2 (Hcp2) expression and secretion (↓); VPA1027-1024 transcript (↓); VPA1027-1024 transcript (↓); VPA1027-1024 transcript (↓); VPA1043-1028 transcript (↓); VPA1044-1046 transcript (↓)
REG0119	VP1241 (TfoX)	T6SS00044 ; T6SS00043	protein	QS regulatory pathway; c-di-GMP regulatory pathway	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	1309223..1309810 (+)	28898016	T6SS2 (↓); T6SS1 (↓)
REG0120	CV_RS20220 (CviR)	T6SS00049	protein	QS regulatory pathway	Chromobacterium violaceum ATCC 12472	chromosome (NC_005085)	4426186..4427046 (+)	1169634970	CV_RS06990 (VgrG) promotor (↓)
REG0121	CV_RS23145 (CviI)	T6SS00049	protein	QS regulatory pathway	Chromobacterium violaceum ATCC 12472	chromosome (NC_005085)	4426967..4427620 (-)	499450174	CV_RS20220 (CviR) (↓)
REG0122	VF2177 (LitR)	T6SS00084	protein	QS regulatory pathway	Aliivibrio fischeri ES114	chromosome (NC_006840)	2446537..2447142 (+)	59712784	Unknown (↓)
REG0151	Psyr_4339 (ladS)	T6SS00086	protein	QS regulatory pathway	Pseudomonas syringae pv. syringae B728a	chromosome (NC_007005)	5191591..5193585 (-)	639463044	T6SS transcription (↓)
REG0152	Psyr_4408 (retS)	T6SS00086	protein	QS regulatory pathway	Pseudomonas syringae pv. syringae B728a	chromosome (NC_007005)	5270882..5273671 (+)	489494774	T6SS transcription (↑)
REG0155	BTH_II1510 (BtaR1)	T6SS00115	protein	QS regulatory pathway	Burkholderia thailandensis E264	chromosome (NC_007650)	1779070..1779789 (-)	497583165	BTH_I2691/BTH_I2693-99/BTH_I2701-702 (↓)
REG0164	PA14_RS05020 (LadS)	T6SS00150	protein	QS regulatory pathway	Pseudomonas aeruginosa UCBPP-PA14	chromosome (NC_008463)	1069280..1071716 (+)	15599169	Unknown
REG0165	PA14_64230 (RetS)	T6SS00150	protein	QS regulatory pathway	Pseudomonas aeruginosa UCBPP-PA14	chromosome (NC_008463)	5722815..5725643 (+)	489202068	Unknown
REG0166	PA14_45960 (lasR)	T6SS00152 ; T6SS00151 ; T6SS00150	protein	QS regulatory pathway	Pseudomonas aeruginosa UCBPP-PA14	chromosome (NC_008463)	4085339..4086058 (-)	489173455	TssA2 (PA14_43050) promotor (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0167	PA14_51340 (MvfR)	T6SS00152 ; T6SS00151 ; T6SS00150	protein	QS regulatory pathway	Pseudomonas aeruginosa UCBPP-PA14	chromosome (NC_008463)	4562837..4563835 (+)	489176730	Upstream of pqs operon (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0168	PA14_51380 (pqsE)	T6SS00150 ; T6SS00152	protein	QS regulatory pathway	Pseudomonas aeruginosa UCBPP-PA14	chromosome (NC_008463)	4566077..4566982 (-)	489176725	T6SS Transciption (↓)
REG0172	PA14_48150 (QslA)	T6SS00152	protein	QS regulatory pathway	Pseudomonas aeruginosa UCBPP-PA14	chromosome (NC_008463)	4286222..4286536 (+)	489177206	H2-T6SS (PA14_43050) promotor (↑)
REG0195	YPK_RS08345 (YpsR)	T6SS00256	protein	QS regulatory pathway	Yersinia pseudotuberculosis YPIII	chromosome (NC_010465)	1837676..1838413 (+)	488140651	T6SS transcripts (↓)
REG0196	YPK_RS03965 (YtbR)	T6SS00256	protein	QS regulatory pathway	Yersinia pseudotuberculosis YPIII	chromosome (NC_010465)	900476..901219 (+)	488140447	T6SS transcripts (↓)
REG0197	YPK_RS08350 (ypsI)	T6SS00256	protein	QS regulatory pathway	Yersinia pseudotuberculosis YPIII	chromosome (NC_010465)	1838406..1839050 (-)	501053391	T6SS transcripts (↓)
REG0198	YPK_RS03970 (ytbI)	T6SS00256 ; T6SS00260	protein	QS regulatory pathway	Yersinia pseudotuberculosis YPIII	chromosome (NC_010465)	901200..901850 (-)	488144695	T6SS transcripts (↓); T6SS4 transcripts and hcp secretion (↓)
REG0246	ETAE_3474 (fabR)	T6SS00372	protein	QS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	3666488..3667159 (-)	502613311	Unknown (↓)
REG0255	HMPREF1171_RS20340	T6SS00788	protein	QS regulatory pathway	Aeromonas dhakensis strain SSU	chromosome (NZ_JH815591)	4528194..4528817 (-)	WP_005308454.1	ahyR (↓)
REG0256	HMPREF1171_RS20345	T6SS00788	protein	QS regulatory pathway	Aeromonas dhakensis strain SSU	chromosome (NZ_JH815591)	4528880..4529662 (+)	WP_005308457.1	hcp and vgrG2 expression (↓)
REG0260	EPGS_00471 (VtsR)	T6SS00794	protein	QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	-	-	LuxO (↓)
REG0261	EPGS_00471 (VtsR)	T6SS00793	protein	QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790776)	-	-	LuxO (↑)
REG0262	luxR	T6SS00794	protein	QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	-	-	T6SS2 transcript and expression (↓)
REG0263	luxR	T6SS00793	protein	QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790776)	-	-	T6SS1 transcript and expression (↑)
REG0264	VEPGS_0621 (pppA)	T6SS00793	protein	Tpp; QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	23577..24245 (+)	225382187	Unknown (↑); T6SS1 transcript (↑); hcp1 transcript (↓)
REG0265	luxS	T6SS00793	protein	QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790776)	-	-	hcp1 transcript (↓)
REG0266	LuxO	T6SS00793	protein	QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790776)	-	-	hcp1 transcript (↓)
REG0267	LuxO	T6SS00794	protein	QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	-	-	Unknown (↑)
REG0275	V12G01_21538 (LuxR)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000053)	13551..14165 (-)	91187814	T6SS2 transcripts (↓); V12G01-18672 (vqsA) promotor
REG0279	V12G01_18672 (vqsA)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000003)	114208..115086 (+)	91191289	V12G01-21538 (luxR) promoter (↓); V12G01-14830 (aphA) promoter (↓); V12G01-18672 (vqsA) promotor (↓)
REG0280	V12G01_10813 (AsnC)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000004)	181050..181484 (-)	91191052	V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0281	V12G01_02325 (HP)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000004)	115263..116411 (+)	91190993	V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0282	V12G01_01665 (HP)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000007)	185299..185712 (+)	91190406	V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0283	V12G01_06711 (HP)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000024)	47707..48585 (-)	91188835	V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0284	V12G01_16177 (MetR)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000023)	58420..59331 (-)	91188901	V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0285	V12G01_02620 (SoxR)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000004)	181050..181484 (-)	91191052	V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0287	V12G01_14830 (aphA)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000027)	42305..42880 (+)	91188695	V12G01-18672 (vqsA) promotor
REG0296	VCA1552_RS03895 (LuxO)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	818142..819509 (+)	487814784	Hcp expression and secretion (↑)
REG0297	VCA1552_RS17020 (cqsa)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024868)	455238..456407 (-)	446962656	Hcp expression and secretion (↓)
REG0298	VCA1552_RS01665 (LuxS)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	319588..320106 (+)	447052971	Hcp expression and secretion (↓)
REG0299	VCA1552_RS00505 (hfq)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	92673..92936 (+)	446974616	VCA1552_00351 (HapR) (↑)
REG0301	VCA1552_RS12025 (crp)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2558371..2559003 (+)	446164894	Hcp expression and secretion (↓)
REG0302	VCA1552_RS12925 (cya)	T6SS01112	protein	QS regulatory pathway; cAMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2747346..2749877 (-)	447072406	VCA1552_RS12025 (crp) (↓)
REG0303	VCA1552_00154 (QstR)	T6SS01112	protein	c-di-GMP regulatory pathway; QS regulatory pathway; Global regulator	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	149920..150564 (-)	447111060	TssB/TfoY transcripts (↓); VC0396/VCA1552_00154 (QstR) (↓); T6SS transcripts (↓)
REG0321	GT391_RS06470 (expR1)	T6SS01170	protein	QS regulatory pathway	Pectobacterium brasiliense strain 1692	chromosome (NZ_CP047495)	1453704..1455596 (+)	746272689	T6SS transcripts (↓)
REG0322	GT391_RS06405 (expI)	T6SS01170	protein	QS regulatory pathway	Pectobacterium brasiliense strain 1692	chromosome (NZ_CP047495)	1436931..1437581 (-)	497970044	GT391_RS06470 (expR1) (↓)
REG0324	WQ49_05050 (cepR)	T6SS01276	protein	QS regulatory pathway	Burkholderia cenocepacia strain K56-2	chromosome (LAUA01000002)	545988..546707 (-)	815852483	T6SS promotor (P (BCAL0340) ) (↓)
REG0325	WQ49_20245 (cciR)	T6SS01276	protein	QS regulatory pathway	Burkholderia cenocepacia strain K56-2	chromosome (LAUA01000016)	843677..844378 (+)	815850423	Unknown (↑)
REG0327	WQ49_05060 (cepI)	T6SS01276	protein	QS regulatory pathway	Burkholderia cenocepacia strain K56-2	chromosome (LAUA01000002)	547435..548043 (+)	815852485	T6SS expression (↓)
REG0328	WQ49_20250 (cciI)	T6SS01276	protein	QS regulatory pathway	Burkholderia cenocepacia strain K56-2	chromosome (LAUA01000016)	844665..845255 (+)	815850424	T6SS expression (↓)
REG0344	BN49_RS26620 (hfq)	T6SS01214	protein	QS regulatory pathway	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	5173711..5174019 (-)	488974767	TssB transcription (↓)
REG0353	AHA_1842 (VasH)	T6SS00157	protein	QS regulatory pathway	Aeromonas hydrophila subsp. hydrophila ATCC 7966	chromosome (NC_008570)	2010521..2012059 (+)	117617496	T6SS gene cluster promoter (PAHA1826) (↓)
REG0355	N779_15250 (VtpR)	T6SS01280 ; T6SS01281	protein	QS regulatory pathway	Vibrio coralliilyticus OCN008	chromosome (AVOO01000045)	7579..8184 (-)	536747482	Unknown (↓)
REG0367	H78_RS02920 (Hfq)	-	protein	QS regulatory pathway	Pseudomonas protegens strain H78	chromosome (NZ_CP013184)	662937..663197 (+)	495196588	T6SS transcription level (↓)
REG0373	hapR	T6SS01028 ; T6SS01029	protein	QS regulatory pathway	Vibrio fluvialis 85003	chromosome (KY319184)	-	-	vgrG (↓)
REG0374	ihfA	T6SS01028 ; T6SS01029	protein	QS regulatory pathway	Vibrio fluvialis 85003	chromosome (KY319184)	-	-	vgrG (↓)
REG0375	ihfB	T6SS01028 ; T6SS01029	protein	QS regulatory pathway	Vibrio fluvialis 85003	chromosome (KY319184)	-	-	vgrG (↓)