Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00150  |
| T6SS type | Type i3 |
| Strain | Pseudomonas aeruginosa UCBPP-PA14 |
| Replicon (RefSeq) | chromosome (NC_008463) |
| Location | 82730..121410 |
| Function | anti-eukaryotic; anti-bacterial; effector translocation; virulence |
| Comment | Important for resistance of biofilms to a subset of antibiotics; mediating P. aeruginosa pathogenesis. |
| References | [1] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] [2] Zhang L et al (2011) Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 193(19):5510-3. [PudMed:21784934] [3] Jones C et al (2013) An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402] [4] Mikkelsen H et al (2011) The Pseudomonas aeruginosa reference strain PA14 displays increased virulence due to a mutation in ladS. PLoS One. 6(12):e29113. [PudMed:22216178] [5] Hachani A et al (2014) The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] [6] Lesic B et al (2009) Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | PpkA | PppA | TagF | TagJ | TagQ | TagR | TagS | TagT | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| PA14_RS00345 | 78396..78671 | + | 276 | WP_003083624.1 | YheV family putative metal-binding protein | ||
| PA14_RS00350 | 78760..79818 | + | 1059 | WP_003083628.1 | PA0069 family radical SAM protein | ||
| T6CP012453 | TagQ | PA14_RS00355 | 80049..80963 | - | 915 | WP_003083630.1 | type VI secretion system-associated lipoprotein TagQ |
| T6CP012454 | TagR | PA14_RS00360 | 81025..82737 | - | 1713 | WP_003083632.1 | type VI secretion system-associated regulator TagR |
| T6CP012455 | TagS | PA14_RS00365 | 82730..83929 | - | 1200 | WP_003136958.1 | type IV secretion associated ABC transporter permease TagS |
| T6CP012456 | TagT | PA14_RS00370 | 83929..84648 | - | 720 | WP_003136959.1 | type IV secretion associated ABC transporter ATP-binding protein TagT |
| T6CP012459 | PpkA | PA14_RS00375 (ppkA) | 84645..87743 | - | 3099 | WP_003136961.1 | serine/threonine protein kinase PpkA |
| T6CP012457 | PppA | PA14_RS00380 | 87751..88479 | - | 729 | WP_003083639.1 | serine/threonine phosphatase PppA |
| T6CP012458 | TagF | PA14_RS00385 (tagF) | 88489..89169 | - | 681 | WP_003083642.1 | type VI secretion system-associated protein TagF |
| T6CP001455 | TssM | PA14_RS00390 (tssM) | 89166..92699 | - | 3534 | WP_003111851.1 | type VI secretion system membrane subunit TssM |
| T6CP001456 | TssL | PA14_RS00395 | 92696..94045 | - | 1350 | WP_003115051.1 | DotU family type VI secretion system protein |
| T6CP001457 | TssK | PA14_RS00400 (tssK) | 94052..95386 | - | 1335 | WP_003115052.1 | type VI secretion system baseplate subunit TssK |
| T6CP001458 | TssJ | PA14_RS00405 (tssJ) | 95402..95866 | - | 465 | WP_003083660.1 | type VI secretion system lipoprotein TssJ |
| PA14_RS00410 (tagH) | 95911..97409 | - | 1499 | pseudogene | type VI secretion system-associated FHA domain protein TagH | ||
| T6CP001459 | TssA | PA14_RS00415 (tssA) | 97777..98811 | + | 1035 | WP_003111627.1 | type VI secretion system protein TssA |
| T6CP001460 | TssB | PA14_RS00420 (tssB) | 98900..99418 | + | 519 | WP_003083666.1 | type VI secretion system contractile sheath small subunit |
| T6CP001461 | TssC | PA14_RS00425 (tssC) | 99431..100927 | + | 1497 | WP_003111626.1 | type VI secretion system contractile sheath large subunit |
| T6CP001462 | TssD | PA14_RS00430 | 101003..101491 | + | 489 | WP_003083670.1 | type VI secretion system receptor/chaperone Hcp |
| T6CP012732 | TagJ | PA14_RS00435 | 101659..102504 | + | 846 | WP_003119488.1 | type VI secretion system accessory protein TagJ |
| T6CP009593 | TssE | PA14_RS00440 (tssE) | 102506..103015 | + | 510 | WP_003111625.1 | type VI secretion system baseplate subunit TssE |
| T6CP001463 | TssF | PA14_RS00445 (tssF) | 103012..104871 | + | 1860 | WP_003083676.1 | type VI secretion system baseplate subunit TssF |
| T6CP001464 | TssG | PA14_RS00450 (tssG) | 104835..105881 | + | 1047 | WP_003104969.1 | type VI secretion system baseplate subunit TssG |
| T6CP001465 | TssH | PA14_RS00455 (tssH) | 105874..108582 | + | 2709 | WP_003136970.1 | type VI secretion system ATPase TssH |
| T6CP001466 | TssI | PA14_RS00460 (vgrG1a) | 108629..110560 | + | 1932 | WP_011666527.1 | type VI secretion system spike protein VgrG1a |
| PA14_RS00465 | 110675..110959 | - | 285 | WP_003111794.1 | hypothetical protein | ||
| PA14_RS00470 (tis1) | 110972..111205 | - | 234 | WP_003083689.1 | T6SS immunity factor Tis1 | ||
| PA14_RS00475 (tas1) | 111195..112577 | - | 1383 | WP_003083691.1 | T6SS effector (p)ppApp synthetase Tas1 | ||
| PA14_RS00480 | 112593..113027 | - | 435 | WP_003083692.1 | DcrB-related protein | ||
| T6CP001467 | TssI | PA14_RS00485 (vgrG1b) | 113281..115506 | + | 2226 | WP_011666529.1 | type VI secretion system spike protein VgrG1b |
| PA14_RS00490 | 115534..115983 | + | 450 | WP_003136987.1 | hypothetical protein | ||
| PA14_RS00495 | 115913..117112 | + | 1200 | WP_003136988.1 | DUF2169 domain-containing protein | ||
| PA14_RS00500 | 117109..118146 | + | 1038 | WP_003136990.1 | 3-oxoacyl-ACP synthase | ||
| PA14_RS00505 | 118146..119237 | + | 1092 | WP_003136992.1 | DUF4150 domain-containing protein | ||
| PA14_RS00510 | 119248..120168 | + | 921 | WP_003136994.1 | hypothetical protein | ||
| PA14_RS00515 | 120178..121410 | + | 1233 | WP_003136996.1 | TIGR02270 family protein | ||
| PA14_RS00520 | 121786..122514 | + | 729 | WP_003083701.1 | carbonic anhydrase | ||
| PA14_RS00525 | 122725..124296 | + | 1572 | WP_003136999.1 | SulP family inorganic anion transporter | ||
| PA14_RS00530 | 124283..124921 | - | 639 | WP_016254346.1 | hypothetical protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed. Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01038 |
PA14_01140 (Tas1) | Tas | IMU00310 |
Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 111195..112577 (-) | 116053814 |
| EFF01816 |
PA14_01200 (Tse7) | Tde | - | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 118146..119237 (+) | 489228633 |
| EFF00183 |
PA14_01030 (hcp1) | - | - | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 101003..101491 (+) | 489174134 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00310 |
PA14_01130 (Tis1) | EFF01038 |
Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 110972..111205 (-) | 489174153 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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