Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00150 experimental
T6SS type Type i3
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon (RefSeq) chromosome (NC_008463)
Location 82730..121410
Function anti-eukaryotic; anti-bacterial; effector translocation; virulence
Comment Important for resistance of biofilms to a subset of antibiotics; mediating P. aeruginosa pathogenesis.
References
[1] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
[2] Zhang L et al (2011) Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 193(19):5510-3. [PudMed:21784934]
[3] Jones C et al (2013) An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402]
[4] Mikkelsen H et al (2011) The Pseudomonas aeruginosa reference strain PA14 displays increased virulence due to a mutation in ladS. PLoS One. 6(12):e29113. [PudMed:22216178]
[5] Hachani A et al (2014) The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869]
[6] Lesic B et al (2009) Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentPpkAPppATagFTagJTagQTagRTagSTagTTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number111111111111111121111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
PA14_RS00345 78396..78671 + 276 WP_003083624.1 YheV family putative metal-binding protein
PA14_RS00350 78760..79818 + 1059 WP_003083628.1 PA0069 family radical SAM protein
T6CP012453 TagQ PA14_RS00355 80049..80963 - 915 WP_003083630.1 type VI secretion system-associated lipoprotein TagQ
T6CP012454 TagR PA14_RS00360 81025..82737 - 1713 WP_003083632.1 type VI secretion system-associated regulator TagR
T6CP012455 TagS PA14_RS00365 82730..83929 - 1200 WP_003136958.1 type IV secretion associated ABC transporter permease TagS
T6CP012456 TagT PA14_RS00370 83929..84648 - 720 WP_003136959.1 type IV secretion associated ABC transporter ATP-binding protein TagT
T6CP012459 PpkA PA14_RS00375 (ppkA) 84645..87743 - 3099 WP_003136961.1 serine/threonine protein kinase PpkA
T6CP012457 PppA PA14_RS00380 87751..88479 - 729 WP_003083639.1 serine/threonine phosphatase PppA
T6CP012458 TagF PA14_RS00385 (tagF) 88489..89169 - 681 WP_003083642.1 type VI secretion system-associated protein TagF
T6CP001455 TssM PA14_RS00390 (tssM) 89166..92699 - 3534 WP_003111851.1 type VI secretion system membrane subunit TssM
T6CP001456 TssL PA14_RS00395 92696..94045 - 1350 WP_003115051.1 DotU family type VI secretion system protein
T6CP001457 TssK PA14_RS00400 (tssK) 94052..95386 - 1335 WP_003115052.1 type VI secretion system baseplate subunit TssK
T6CP001458 TssJ PA14_RS00405 (tssJ) 95402..95866 - 465 WP_003083660.1 type VI secretion system lipoprotein TssJ
PA14_RS00410 (tagH) 95911..97409 - 1499 pseudogene type VI secretion system-associated FHA domain protein TagH
T6CP001459 TssA PA14_RS00415 (tssA) 97777..98811 + 1035 WP_003111627.1 type VI secretion system protein TssA
T6CP001460 TssB PA14_RS00420 (tssB) 98900..99418 + 519 WP_003083666.1 type VI secretion system contractile sheath small subunit
T6CP001461 TssC PA14_RS00425 (tssC) 99431..100927 + 1497 WP_003111626.1 type VI secretion system contractile sheath large subunit
T6CP001462 TssD PA14_RS00430 101003..101491 + 489 WP_003083670.1 type VI secretion system receptor/chaperone Hcp
T6CP012732 TagJ PA14_RS00435 101659..102504 + 846 WP_003119488.1 type VI secretion system accessory protein TagJ
T6CP009593 TssE PA14_RS00440 (tssE) 102506..103015 + 510 WP_003111625.1 type VI secretion system baseplate subunit TssE
T6CP001463 TssF PA14_RS00445 (tssF) 103012..104871 + 1860 WP_003083676.1 type VI secretion system baseplate subunit TssF
T6CP001464 TssG PA14_RS00450 (tssG) 104835..105881 + 1047 WP_003104969.1 type VI secretion system baseplate subunit TssG
T6CP001465 TssH PA14_RS00455 (tssH) 105874..108582 + 2709 WP_003136970.1 type VI secretion system ATPase TssH
T6CP001466 TssI PA14_RS00460 (vgrG1a) 108629..110560 + 1932 WP_011666527.1 type VI secretion system spike protein VgrG1a
PA14_RS00465 110675..110959 - 285 WP_003111794.1 hypothetical protein
PA14_RS00470 (tis1) 110972..111205 - 234 WP_003083689.1 T6SS immunity factor Tis1
PA14_RS00475 (tas1) 111195..112577 - 1383 WP_003083691.1 T6SS effector (p)ppApp synthetase Tas1
PA14_RS00480 112593..113027 - 435 WP_003083692.1 DcrB-related protein
T6CP001467 TssI PA14_RS00485 (vgrG1b) 113281..115506 + 2226 WP_011666529.1 type VI secretion system spike protein VgrG1b
PA14_RS00490 115534..115983 + 450 WP_003136987.1 hypothetical protein
PA14_RS00495 115913..117112 + 1200 WP_003136988.1 DUF2169 domain-containing protein
PA14_RS00500 117109..118146 + 1038 WP_003136990.1 3-oxoacyl-ACP synthase
PA14_RS00505 118146..119237 + 1092 WP_003136992.1 DUF4150 domain-containing protein
PA14_RS00510 119248..120168 + 921 WP_003136994.1 hypothetical protein
PA14_RS00515 120178..121410 + 1233 WP_003136996.1 TIGR02270 family protein
PA14_RS00520 121786..122514 + 729 WP_003083701.1 carbonic anhydrase
PA14_RS00525 122725..124296 + 1572 WP_003136999.1 SulP family inorganic anion transporter
PA14_RS00530 124283..124921 - 639 WP_016254346.1 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0157 experimental rmsF protein RsmA regulatory pathway Pseudomonas aeruginosa PA103 chromosome (NZ_JARI00000000) - - tssA1 RNA (↑); RsmY/Z (↑)
REG0158 experimental PA14_52570 (RsmA) protein RsmA regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4660393..4660578 (-) 489176459 RSMF (↑); tssA1 RNA (↑); RSMA (↑)
REG0159 experimental PA14_RS20260/PA1120 (YfiN/TpbB) protein c-di-GMP regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4435431..4436738 (-) 489207392 Hcp1 production (↓)
REG0160 experimental PA14_RS15060 (PA2133 phosphodiesterase) protein c-di-GMP regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 3294000..3294857 (-) 489200657 Hcp1 production (↑)
REG0161 experimental PA14_RS06615 (WspR) protein c-di-GMP regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1413158..1414201 (+) 489183089 Hcp1 production (↓)
REG0162 experimental RsmY ncRNA RsmA regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 596840..596963 (+) Unknown
REG0163 experimental RsmZ ncRNA RsmA regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1500352..1500467 (+) Unknown
REG0164 experimental PA14_RS05020 (LadS) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1069280..1071716 (+) 15599169 Unknown
REG0165 experimental PA14_64230 (RetS) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 5722815..5725643 (+) 489202068 Unknown
REG0166 experimental PA14_45960 (lasR) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4085339..4086058 (-) 489173455 TssA2 (PA14_43050) promotor (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0167 experimental PA14_51340 (MvfR) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4562837..4563835 (+) 489176730 Upstream of pqs operon (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0168 experimental PA14_51380 (pqsE) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4566077..4566982 (-) 489176725 T6SS Transciption (↓)
REG0169 experimental PA14_22470 (PA3225) protein Global regulator Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1955624..1956553 (-) 489182081 PA14_00990 (TssA) promotor (↓); TssA1 (PA14_00990) transcript (↑); TssA2 (PA14_43050) transcript (↑); TssB3 (PA14_34070) transcript (↑)
REG0170 experimental PA14_20290 (AmrZ) protein Global regulator Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1747602..1747928 (-) 489182353 TssA1 (PA14_00990) promotor (↓); TssA2 (PA14_43050) promotor (↑); TssB3 (PA14_34070) promotor (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01038 experimental PA14_01140 (Tas1) Tas IMU00310 experimental Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 111195..112577 (-) 116053814
EFF01816 experimental PA14_01200 (Tse7) Tde - Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 118146..119237 (+) 489228633
EFF00183 experimental PA14_01030 (hcp1) - - Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 101003..101491 (+) 489174134

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00310 experimental PA14_01130 (Tis1) EFF01038 experimental Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 110972..111205 (-) 489174153

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector