Detailed information of regulator
Summary
| Regulator ID | REG0159  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | c-di-GMP regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00150 (Type i3) |
| Strain | Pseudomonas aeruginosa UCBPP-PA14 |
| Replicon | chromosome |
| Sequence | Protein sequence (436 a.a.); Nucleotide sequence (1308 bp) |
| Description | Overexpression of PA1120 in this strain resulted in a switch between the T3SS and the T6SS, similar to the one observed upon overexpression of wspRR242A. |
| Reference |
External database links
| Locus tag (Gene) | PA14_RS20260/PA1120 (YfiN/TpbB) |
| Coordinates (Strand) | 4435431..4436738 (-) |
| NCBI ID | 489207392 |
| RefSeq | NC_008463 |
| Uniprot ID | A0A1C7BNH9_PSEAI, A0A0H2Z7X0_PSEAB, A0A0T6KSV7_PSEAI |
| KEGG ID | pau:PA14_49890 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Hcp1 production | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-435 | LLGWSLWGSTVYLLFFLKDAGVVEFRQAPAAAGLALGDVHRHVGVVEQRRRGIRVLGIEGDADGQRGTHRAAVAEPDRRLHAGDDVVGDAQGVGAGRQRCEQDGEFVATQARHQVAFAQLPTDAHGDVDQHGVAGGMAEAVVDLLETVAVDEQYRQLLAVLAGIAQRPAQAAFEEGAVGQAGQAVVVGLVRQRGVLVLQARLPGFEFVEQGVEVLAQLAQLGDAGFRYALLEIPFAAHGMRDLRQLVERADDTAYQPP*QVEGAQGGKQQAQDHAGETGEQEAQQAAAGAFHLHRTDLLAVVQDRLFGRRAEQPVRQLLFQQVQRPGAFPMPAGQGLPLAVVDDGAGDLFAGGDQRQGFLGGLGIAEDHRGFDGIADGTGDQLLVGVRVGAQGQQADQGHCQPGGEYRDQRHAQVRAIEHPAQRWIGTRIATTTV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9190 | - | - |
Protein sequence of regulator: 436 a.a. .
>REG0159 NC_008463:c4436738-4435431 [Pseudomonas aeruginosa UCBPP-PA14]
LLGWSLWGSTVYLLFFLKDAGVVEFRQAPAAAGLALGDVHRHVGVVEQRRRGIRVLGIEGDADGQRGTHRAAVAEPDRRL
HAGDDVVGDAQGVGAGRQRCEQDGEFVATQARHQVAFAQLPTDAHGDVDQHGVAGGMAEAVVDLLETVAVDEQYRQLLAV
LAGIAQRPAQAAFEEGAVGQAGQAVVVGLVRQRGVLVLQARLPGFEFVEQGVEVLAQLAQLGDAGFRYALLEIPFAAHGM
RDLRQLVERADDTAYQPP*QVEGAQGGKQQAQDHAGETGEQEAQQAAAGAFHLHRTDLLAVVQDRLFGRRAEQPVRQLLF
QQVQRPGAFPMPAGQGLPLAVVDDGAGDLFAGGDQRQGFLGGLGIAEDHRGFDGIADGTGDQLLVGVRVGAQGQQADQGH
CQPGGEYRDQRHAQVRAIEHPAQRWIGTRIATTTVH
LLGWSLWGSTVYLLFFLKDAGVVEFRQAPAAAGLALGDVHRHVGVVEQRRRGIRVLGIEGDADGQRGTHRAAVAEPDRRL
HAGDDVVGDAQGVGAGRQRCEQDGEFVATQARHQVAFAQLPTDAHGDVDQHGVAGGMAEAVVDLLETVAVDEQYRQLLAV
LAGIAQRPAQAAFEEGAVGQAGQAVVVGLVRQRGVLVLQARLPGFEFVEQGVEVLAQLAQLGDAGFRYALLEIPFAAHGM
RDLRQLVERADDTAYQPP*QVEGAQGGKQQAQDHAGETGEQEAQQAAAGAFHLHRTDLLAVVQDRLFGRRAEQPVRQLLF
QQVQRPGAFPMPAGQGLPLAVVDDGAGDLFAGGDQRQGFLGGLGIAEDHRGFDGIADGTGDQLLVGVRVGAQGQQADQGH
CQPGGEYRDQRHAQVRAIEHPAQRWIGTRIATTTVH
Nucleotide sequence of regulator: 1308 bp .
>REG0159 NC_008463:c4436738-4435431 [Pseudomonas aeruginosa UCBPP-PA14]
CTACTTGGGTGGAGCCTCTGGGGTAGCACTGTCTATCTCCTTTTCTTCCTGAAGGATGCGGGGGTCGTTGAGTTCCGCCA
GGCGCCGGCTGCCGCGGGCCTGGCGCTTGGCGATGTACATCGCCATGTCGGCGTCGTGGAGCAGCGCCGCCGGGGTATCC
GCGTGCTCGGGATAGAGGGCGATGCCGATGGTCAGCGAGGTACTCACCGTGCTGCCGTCGCTGAGCCGGATCGGCGCCTG
CATGCTGGCGATGATGTTGTCGGCGATGCGCAGGGCGTCGGCGCCGGACGCCAGCGGTGCGAGCAGGACGGCGAATTCGT
CGCCACCCAGGCGCGCCACCAGGTCGCTTTCGCGCAACTGCCCACGGATGCGCATGGCGATGTTGACCAGCACGGTGTCG
CCGGCGGCATGGCCGAGGCGGTCGTTGATCTCCTTGAAACGGTCGCTGTCGATGAACAGTACCGCCAATTGCTCGCGGTG
CTCGCTGGCATCGCGCAGCGCCCGGCTCAGGCGGCCTTCGAAGAAGGCGCGGTTGGGCAGGCTGGTCAGGCTGTCGTGGT
GGGCCTGGTGCGCCAGCGAGGCGTTCTCGTCCTGCAAGCGCGCCTGCCAGGATTCGAGTTCGTCGAGCAGGGCGTTGAAG
TCCTCGCCCAGTTGGCTCAGCTCGGCGATGCCGGCTTCCGGTACGCGCTTCTCGAAATCCCGTTCGCGGCGCACGGTATG
CGCGACCTTCGCCAGTTGGTCGAGCGGGCCGACGATACCGCGTACCAGCCGCCGTGACAGGTAGAAGGCGCCCAGGGCGG
TAAGCAGCAGGCACAGGACCATGCCGGCGAAACCGGTGAGCAGGAAGCGCAGCAGGCTGCCGCCGGAGCCTTTCACCTCC
ACCGAACCGATCTTCTGGCCGTCGTGCAGGATCGGTTGTTCGGTCGGCGCGCTGAGCAGCCAGTGCGCCAGTTGCTGTTC
CAGCAGGTGCAGCGGCCCGGTGCTTTCCCGATGCCAGCTGGCCAGGGGCTGCCCCTGGCGGTCGTAGACGATGGCGCTGG
AGACCTCTTCGCTGGAGGCGATCAGCGCCAGGGATTCCTCGGCGGCCTGGGCATCGCCGAAGACCACCGCGGCTTCGACG
GTATAGCTGATGGAACGGGCGATCAGTTGCTGGTTGGGGTCCGCGTAGGCGCGCAGGGTCAGCAGGCCGACCAGGGTCAC
TGCCAGCCCGGCGGTGAATACCGCGACCAGCGCCACGCCCAGGTGCGCGCGATAGAGCACCCGGCGCAGCGATGGATTGG
AACCCGTATAGCGACGACGACGGTTCAT
CTACTTGGGTGGAGCCTCTGGGGTAGCACTGTCTATCTCCTTTTCTTCCTGAAGGATGCGGGGGTCGTTGAGTTCCGCCA
GGCGCCGGCTGCCGCGGGCCTGGCGCTTGGCGATGTACATCGCCATGTCGGCGTCGTGGAGCAGCGCCGCCGGGGTATCC
GCGTGCTCGGGATAGAGGGCGATGCCGATGGTCAGCGAGGTACTCACCGTGCTGCCGTCGCTGAGCCGGATCGGCGCCTG
CATGCTGGCGATGATGTTGTCGGCGATGCGCAGGGCGTCGGCGCCGGACGCCAGCGGTGCGAGCAGGACGGCGAATTCGT
CGCCACCCAGGCGCGCCACCAGGTCGCTTTCGCGCAACTGCCCACGGATGCGCATGGCGATGTTGACCAGCACGGTGTCG
CCGGCGGCATGGCCGAGGCGGTCGTTGATCTCCTTGAAACGGTCGCTGTCGATGAACAGTACCGCCAATTGCTCGCGGTG
CTCGCTGGCATCGCGCAGCGCCCGGCTCAGGCGGCCTTCGAAGAAGGCGCGGTTGGGCAGGCTGGTCAGGCTGTCGTGGT
GGGCCTGGTGCGCCAGCGAGGCGTTCTCGTCCTGCAAGCGCGCCTGCCAGGATTCGAGTTCGTCGAGCAGGGCGTTGAAG
TCCTCGCCCAGTTGGCTCAGCTCGGCGATGCCGGCTTCCGGTACGCGCTTCTCGAAATCCCGTTCGCGGCGCACGGTATG
CGCGACCTTCGCCAGTTGGTCGAGCGGGCCGACGATACCGCGTACCAGCCGCCGTGACAGGTAGAAGGCGCCCAGGGCGG
TAAGCAGCAGGCACAGGACCATGCCGGCGAAACCGGTGAGCAGGAAGCGCAGCAGGCTGCCGCCGGAGCCTTTCACCTCC
ACCGAACCGATCTTCTGGCCGTCGTGCAGGATCGGTTGTTCGGTCGGCGCGCTGAGCAGCCAGTGCGCCAGTTGCTGTTC
CAGCAGGTGCAGCGGCCCGGTGCTTTCCCGATGCCAGCTGGCCAGGGGCTGCCCCTGGCGGTCGTAGACGATGGCGCTGG
AGACCTCTTCGCTGGAGGCGATCAGCGCCAGGGATTCCTCGGCGGCCTGGGCATCGCCGAAGACCACCGCGGCTTCGACG
GTATAGCTGATGGAACGGGCGATCAGTTGCTGGTTGGGGTCCGCGTAGGCGCGCAGGGTCAGCAGGCCGACCAGGGTCAC
TGCCAGCCCGGCGGTGAATACCGCGACCAGCGCCACGCCCAGGTGCGCGCGATAGAGCACCCGGCGCAGCGATGGATTGG
AACCCGTATAGCGACGACGACGGTTCAT