Detailed information of component protein


Summary

Component ID T6CP012732
Component protein type TagJ
T6SS ID (Type) T6SS00150 experimental (Type i3)
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon chromosome
Sequence Protein sequence (282 a.a.); Nucleotide sequence (846 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Zhang L, Hinz AJ, Nadeau JP, et al. Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 2011 Oct;193(19):5510-3. doi: 10.1128/JB.00268-11. Epub 2011 Jul 22. PMID: 21784934
[3] Lesic B, Starkey M, He J, et al. Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology (Reading). 2009 Sep;155(Pt 9):2845-2855. doi: 10.1099/mic.0.029082-0. Epub 2009 Jun 4. PMID: 19497948
[4] Mikkelsen H, McMullan R, Filloux A. The Pseudomonas aeruginosa reference strain PA14 displays increased virulence due to a mutation in ladS. PLoS One. 2011;6(12):e29113. doi: 10.1371/journal.pone.0029113. Epub 2011 Dec 22. PMID: 22216178
[5] Hachani A, Allsopp LP, Oduko Y, et al. The VgrG proteins are "à la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 2014 Jun 20;289(25):17872-84. doi: 10.1074/jbc.M114.563429. Epub 2014 May 2. PMID: 24794869
[6] Jones C, Hachani A, Manoli E, et al. An rhs gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 2014 Feb;196(4):800-10. doi: 10.1128/JB.00863-13. Epub 2013 Dec 6. PMID: 24317402
[7] Zhang L, Hinz AJ, Nadeau JP, et al. Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 2011 Oct;193(19):5510-3. doi: 10.1128/JB.00268-11. Epub 2011 Jul 22. PMID: 21784934
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PA14_RS00435
Coordinate (Strand) 101659..102504 (+)
NCBI ID WP_003119488.1
RefSeq NC_008463
Uniprot ID A0A0H2ZK14_PSEAB, TAGJ_PSEAE, A0A6N0KU25_9PSED, A0A2V3DCX1_PSEAI
KEGG ID pau:PA14_01040, pae:PA0086
PDB ID 4UQZ, 4UQX, 4UQY
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpE PF07024.15 1.5e-43 159..274
TPR_19 PF14559.8 2.8e-06 26..82
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-281 MADPSFASGRLGSRLQGSIAMIAEELLRAGRLDDALKALQEQVRSQPSNATLRIFLFQLLAVMGQWARAQNQLKVVGELDASALPMVQTYSTAIDCEALRREVFAGRLTPVILGQPAEWIAPLLQALSLDAEGHGEAAQALREQAFDAAPAVPGRIGEAPFAWLADADTRLGPVLEVIVNGRYAWLPMSNLRSLKVEAPSDLRDLVWLPAELTLANGGATVALLPARYAETVEHGDDAARLGRKTEWLDSGLPVGQRLFVTDAGETALFDLRELDFEPTDA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9704 - -


  Protein sequence: 282 a.a.    

>T6CP012732 NC_008463:101659-102504 [Pseudomonas aeruginosa UCBPP-PA14] [TagJ]
MADPSFASGRLGSRLQGSIAMIAEELLRAGRLDDALKALQEQVRSQPSNATLRIFLFQLLAVMGQWARAQNQLKVVGELD
ASALPMVQTYSTAIDCEALRREVFAGRLTPVILGQPAEWIAPLLQALSLDAEGHGEAAQALREQAFDAAPAVPGRIGEAP
FAWLADADTRLGPVLEVIVNGRYAWLPMSNLRSLKVEAPSDLRDLVWLPAELTLANGGATVALLPARYAETVEHGDDAAR
LGRKTEWLDSGLPVGQRLFVTDAGETALFDLRELDFEPTDA*

  Nucleotide sequence: 846 bp    

>T6CP012732 NC_008463:101659-102504 [Pseudomonas aeruginosa UCBPP-PA14] [TagJ]
ATGGCCGACCCTTCATTCGCATCCGGGCGCCTGGGCTCCCGGCTACAGGGAAGTATCGCGATGATCGCCGAAGAACTGTT
GCGCGCCGGGCGCCTGGACGATGCCTTGAAAGCCTTGCAGGAACAGGTGCGCAGCCAGCCGTCGAACGCCACCCTGCGGA
TCTTCCTGTTCCAGCTGCTGGCCGTGATGGGCCAGTGGGCGCGGGCGCAGAACCAGCTCAAGGTGGTCGGCGAACTGGAT
GCCTCGGCGCTGCCGATGGTACAGACCTACTCCACCGCCATCGACTGCGAGGCGCTGCGCCGCGAGGTGTTCGCCGGGCG
CCTGACGCCGGTGATCCTCGGCCAGCCGGCGGAATGGATCGCGCCGCTGTTGCAGGCCCTGAGCCTGGACGCCGAGGGCC
ATGGCGAGGCCGCCCAGGCCCTGCGCGAGCAGGCCTTCGACGCCGCGCCGGCGGTGCCCGGGCGGATCGGCGAAGCGCCG
TTCGCCTGGCTGGCAGATGCCGACACGCGCCTCGGCCCGGTGCTCGAGGTAATCGTCAACGGTCGCTATGCCTGGTTGCC
GATGAGCAACCTGCGCAGCCTCAAGGTCGAGGCGCCGAGCGACCTGCGCGACCTGGTCTGGCTGCCGGCCGAACTGACCC
TGGCCAACGGTGGCGCCACCGTCGCGCTGCTGCCGGCGCGCTACGCGGAAACCGTCGAGCACGGCGACGACGCCGCGCGC
CTGGGGCGCAAGACCGAATGGCTGGATAGCGGCCTGCCGGTCGGCCAGCGGCTGTTCGTCACCGATGCCGGCGAGACCGC
GCTGTTCGACCTGCGTGAGCTGGACTTCGAGCCGACGGACGCCTGA