Detailed information of regulator


Summary

Regulator ID REG0161 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00150 experimental (Type i3)
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon chromosome
Sequence Protein sequence (348 a.a.); Nucleotide sequence (1044 bp)
Description The recombinant plasmid overproducing WspRR242A was introduced into PAKDpel, which resulted in a > twofold increase in c-di-GMP levels and this increase was concomitant with an increase in Hcp1 production (T6SS).
Reference
External database links

Locus tag (Gene) PA14_RS06615 (WspR)
Coordinates (Strand) 1413158..1414201 (+)
NCBI ID 489183089
RefSeq NC_008463
Uniprot ID V6ABD6_PSEAI, A0A1C7BJG5_PSEAI, A0A0H2ZEX4_PSEAB, A0A077JNP3_PSEAI
KEGG ID pau:PA14_16500
KEGG pathway (determined by BlastKOALA) map02020; map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Hcp1 production ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
GGDEF PF00990.23 5.2e-50 172..333
Response_reg PF00072.26 4.1e-19 21..133
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-347 MHNPHESKTDLGAPLDGAVMVLLVDDQAMIGEAVRRSLASEAGIDFHFCSDPQQAVAVANQIKPTVILQDLVMPGVDGLTLLAAYRGNPATRDIPIIVLSTKEEPTVKSAAFAAGANDYLVKLPDAIELVARIRYHSRSYIALQQRDEAYRALRESQQQLLETNLVLQRLMNSDGLTGLSNRRHFDEYLEMEWRRSLREQSQLSLLMIDVDYFKSYNDTFGHVAGDEALRQVAGAIREGCSRSSDLAARYGGEEFAMVLPGTSPGGARLLAEKVRRTVESLQISHDQPRPGSYLTVSIGVSTLVPGGGGQTFRVLIEMADQALYQAKNNGRNQVGLMEQPVPPAPAG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9849 - -


  Protein sequence of regulator: 348 a.a.    .

>REG0161 NC_008463:1413158-1414201 [Pseudomonas aeruginosa UCBPP-PA14]
MHNPHESKTDLGAPLDGAVMVLLVDDQAMIGEAVRRSLASEAGIDFHFCSDPQQAVAVANQIKPTVILQDLVMPGVDGLT
LLAAYRGNPATRDIPIIVLSTKEEPTVKSAAFAAGANDYLVKLPDAIELVARIRYHSRSYIALQQRDEAYRALRESQQQL
LETNLVLQRLMNSDGLTGLSNRRHFDEYLEMEWRRSLREQSQLSLLMIDVDYFKSYNDTFGHVAGDEALRQVAGAIREGC
SRSSDLAARYGGEEFAMVLPGTSPGGARLLAEKVRRTVESLQISHDQPRPGSYLTVSIGVSTLVPGGGGQTFRVLIEMAD
QALYQAKNNGRNQVGLMEQPVPPAPAG*

  Nucleotide sequence of regulator: 1044 bp    .

>REG0161 NC_008463:1413158-1414201 [Pseudomonas aeruginosa UCBPP-PA14]
ATGCACAACCCTCATGAGAGCAAGACCGACCTGGGCGCCCCTCTGGACGGCGCGGTCATGGTACTGCTTGTCGATGATCA
GGCCATGATCGGAGAGGCTGTGCGTCGCTCGCTGGCGAGCGAGGCGGGCATCGACTTCCACTTCTGTTCCGATCCGCAGC
AGGCGGTGGCGGTGGCCAACCAGATCAAGCCGACGGTGATCCTCCAGGACCTGGTGATGCCCGGCGTCGACGGCCTCACG
CTGCTCGCCGCCTACCGCGGCAACCCGGCGACCCGCGACATCCCGATCATCGTCCTGTCGACCAAGGAAGAGCCGACGGT
GAAGAGCGCGGCGTTCGCCGCCGGCGCCAACGACTACCTGGTCAAGCTGCCGGACGCCATCGAGCTGGTGGCGCGGATCC
GCTACCACTCGCGTTCGTACATCGCCCTGCAGCAACGCGACGAGGCCTATCGGGCGCTGCGCGAGAGCCAGCAGCAGTTG
CTGGAGACCAACCTGGTGCTGCAGCGGCTGATGAACTCCGACGGCCTGACCGGGCTCTCCAACCGTCGTCACTTCGACGA
ATACCTGGAGATGGAGTGGCGCCGCTCGCTGCGCGAGCAGTCGCAACTGTCGCTGCTGATGATCGACGTGGACTATTTCA
AGAGCTACAACGACACCTTCGGCCACGTCGCCGGCGACGAGGCCCTGCGCCAGGTCGCCGGGGCCATCCGCGAGGGCTGC
AGTCGCTCCTCGGACCTGGCGGCGCGCTATGGCGGCGAGGAGTTCGCCATGGTCCTGCCGGGCACCTCGCCGGGCGGCGC
GCGGCTGCTGGCGGAAAAGGTCCGGCGTACGGTGGAGAGCCTGCAGATCAGCCACGACCAGCCACGCCCGGGCTCGTACC
TGACCGTCAGCATCGGCGTCAGCACCCTGGTGCCGGGCGGCGGCGGACAGACCTTCCGGGTTCTCATCGAGATGGCCGAC
CAGGCGCTCTACCAGGCCAAGAACAATGGCCGCAACCAGGTTGGCCTGATGGAACAGCCGGTGCCGCCAGCCCCGGCGGG
CTGA