Detailed information of component protein
Summary
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | PA14_RS00370 |
| Coordinate (Strand) | 83929..84648 (-) |
| NCBI ID | WP_003136959.1 |
| RefSeq | NC_008463 |
| Uniprot ID | V6A7J3_PSEAI, A0A0H2ZJQ6_PSEAB, A0A6N0KVX1_9PSED, A0A2R4AZF2_PSEAI |
| KEGG ID | pau:PA14_00860 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ABC_tran | PF00005.29 | 1.1e-24 | 26..176 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-239 | MILRLHEVARSHGAGRQRYRLRVPALELRAGRQLAIVGPSGCGKSTLLDLLALALAPDPGGRFEFLAGERPQDIAALWRDGRQDRLAALRRQHCGYVLQSGGLLEFLDVRRNIGLPCALLGAPSAERVQALAERLEIGDQLDKKPAALSVGQRQRVAIARALAHAPRLLLADEPTAALDPLNAERVMRLLVEQARERGACTVVATHDERLARAAGLEVLAIRWQREADDSVGACLEDVG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9143 | - | - |
Protein sequence: 240 a.a.
>T6CP012456 NC_008463:c84648-83929 [Pseudomonas aeruginosa UCBPP-PA14] [TagT]
MILRLHEVARSHGAGRQRYRLRVPALELRAGRQLAIVGPSGCGKSTLLDLLALALAPDPGGRFEFLAGERPQDIAALWRD
GRQDRLAALRRQHCGYVLQSGGLLEFLDVRRNIGLPCALLGAPSAERVQALAERLEIGDQLDKKPAALSVGQRQRVAIAR
ALAHAPRLLLADEPTAALDPLNAERVMRLLVEQARERGACTVVATHDERLARAAGLEVLAIRWQREADDSVGACLEDVG*
MILRLHEVARSHGAGRQRYRLRVPALELRAGRQLAIVGPSGCGKSTLLDLLALALAPDPGGRFEFLAGERPQDIAALWRD
GRQDRLAALRRQHCGYVLQSGGLLEFLDVRRNIGLPCALLGAPSAERVQALAERLEIGDQLDKKPAALSVGQRQRVAIAR
ALAHAPRLLLADEPTAALDPLNAERVMRLLVEQARERGACTVVATHDERLARAAGLEVLAIRWQREADDSVGACLEDVG*
Nucleotide sequence: 720 bp
>T6CP012456 NC_008463:c84648-83929 [Pseudomonas aeruginosa UCBPP-PA14] [TagT]
ATGATCCTGCGCCTGCACGAGGTCGCCCGCAGCCACGGCGCCGGTCGCCAGCGCTACCGCCTGCGGGTGCCGGCGCTGGA
ATTGCGCGCGGGTCGCCAGCTGGCCATCGTCGGCCCCAGCGGCTGCGGCAAGAGCACCCTGCTCGACCTGCTGGCGCTGG
CCCTGGCGCCGGACCCGGGCGGGCGCTTCGAATTCCTCGCCGGGGAACGCCCGCAGGACATCGCCGCGCTCTGGCGCGAC
GGCCGCCAGGACCGCTTGGCGGCGCTGCGCCGGCAGCACTGCGGCTACGTGCTGCAAAGCGGCGGGTTGCTCGAGTTCCT
CGATGTGCGGCGCAACATCGGACTGCCCTGCGCGTTGCTCGGCGCCCCGTCCGCCGAGCGCGTGCAGGCGCTGGCCGAGC
GGCTGGAAATCGGCGACCAGCTGGACAAGAAACCGGCGGCGCTGTCGGTCGGCCAGCGCCAGCGGGTCGCCATCGCCCGC
GCCCTGGCGCATGCGCCGCGCCTGTTGCTGGCCGACGAGCCGACCGCGGCGCTCGACCCGCTGAACGCCGAACGGGTGAT
GCGCCTGCTGGTGGAACAGGCCCGCGAGCGCGGCGCCTGCACCGTGGTCGCCACCCACGACGAGCGCCTGGCCCGCGCCG
CCGGACTCGAAGTCCTGGCCATCCGCTGGCAACGCGAGGCCGACGACAGCGTCGGCGCCTGCCTCGAGGACGTTGGCTGA
ATGATCCTGCGCCTGCACGAGGTCGCCCGCAGCCACGGCGCCGGTCGCCAGCGCTACCGCCTGCGGGTGCCGGCGCTGGA
ATTGCGCGCGGGTCGCCAGCTGGCCATCGTCGGCCCCAGCGGCTGCGGCAAGAGCACCCTGCTCGACCTGCTGGCGCTGG
CCCTGGCGCCGGACCCGGGCGGGCGCTTCGAATTCCTCGCCGGGGAACGCCCGCAGGACATCGCCGCGCTCTGGCGCGAC
GGCCGCCAGGACCGCTTGGCGGCGCTGCGCCGGCAGCACTGCGGCTACGTGCTGCAAAGCGGCGGGTTGCTCGAGTTCCT
CGATGTGCGGCGCAACATCGGACTGCCCTGCGCGTTGCTCGGCGCCCCGTCCGCCGAGCGCGTGCAGGCGCTGGCCGAGC
GGCTGGAAATCGGCGACCAGCTGGACAAGAAACCGGCGGCGCTGTCGGTCGGCCAGCGCCAGCGGGTCGCCATCGCCCGC
GCCCTGGCGCATGCGCCGCGCCTGTTGCTGGCCGACGAGCCGACCGCGGCGCTCGACCCGCTGAACGCCGAACGGGTGAT
GCGCCTGCTGGTGGAACAGGCCCGCGAGCGCGGCGCCTGCACCGTGGTCGCCACCCACGACGAGCGCCTGGCCCGCGCCG
CCGGACTCGAAGTCCTGGCCATCCGCTGGCAACGCGAGGCCGACGACAGCGTCGGCGCCTGCCTCGAGGACGTTGGCTGA