Detailed information of component protein
Summary
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | PA14_RS00385 |
| Coordinate (Strand) | 88489..89169 (-) |
| NCBI ID | WP_003083642.1 |
| RefSeq | NC_008463 |
| Uniprot ID | A0A1C7BGZ3_PSEAI, A0A1F0IK56_9PSED, A0A0H2ZK01_PSEAB, A0A6N0KU35_9PSED, A0A077JJU0_PSEAI |
| KEGG ID | pau:PA14_00900 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TagF_N | PF09867.11 | 6.8e-49 | 5..139 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-226 | MNSVGFYGKLAGRGDFVSRGLPNTFVEPWDAWLASGMRASQDELGAAWLDAYLTSPLWRFAIAPGLLGGEAVAGVVMPSIDRVGRYFPLTVACLLPANADLGGLVGGDDGWFEQVESLLLSTLEPEAEVEAFEQAVAQLPAPPCGPRIEQSLISGNLLRSEAVTPAQRLAALAQHACDGASHWWGRGSARISAGLMRYQGLPPAPAFGRFLTGEGEVIPLFPGIPG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9865 | - | - |
Protein sequence: 227 a.a.
>T6CP012458 NC_008463:c89169-88489 [Pseudomonas aeruginosa UCBPP-PA14] [TagF]
MNSVGFYGKLAGRGDFVSRGLPNTFVEPWDAWLASGMRASQDELGAAWLDAYLTSPLWRFAIAPGLLGGEAVAGVVMPSI
DRVGRYFPLTVACLLPANADLGGLVGGDDGWFEQVESLLLSTLEPEAEVEAFEQAVAQLPAPPCGPRIEQSLISGNLLRS
EAVTPAQRLAALAQHACDGASHWWGRGSARISAGLMRYQGLPPAPAFGRFLTGEGEVIPLFPGIPG*
MNSVGFYGKLAGRGDFVSRGLPNTFVEPWDAWLASGMRASQDELGAAWLDAYLTSPLWRFAIAPGLLGGEAVAGVVMPSI
DRVGRYFPLTVACLLPANADLGGLVGGDDGWFEQVESLLLSTLEPEAEVEAFEQAVAQLPAPPCGPRIEQSLISGNLLRS
EAVTPAQRLAALAQHACDGASHWWGRGSARISAGLMRYQGLPPAPAFGRFLTGEGEVIPLFPGIPG*
Nucleotide sequence: 681 bp
>T6CP012458 NC_008463:c89169-88489 [Pseudomonas aeruginosa UCBPP-PA14] [TagF]
TTGAACAGCGTCGGTTTCTACGGCAAGCTGGCCGGCCGCGGCGATTTCGTCAGCCGCGGCCTGCCGAACACCTTCGTCGA
ACCCTGGGACGCCTGGCTGGCCAGCGGCATGCGCGCCAGCCAGGACGAACTCGGCGCGGCCTGGCTCGACGCCTACCTGA
CCAGCCCGCTGTGGCGCTTCGCCATCGCCCCCGGCCTGCTCGGCGGCGAAGCGGTGGCCGGGGTGGTGATGCCGAGCATC
GACCGGGTCGGTCGCTATTTCCCGCTGACCGTCGCCTGCCTGCTGCCGGCGAACGCCGACCTCGGCGGGCTGGTCGGCGG
CGACGACGGCTGGTTCGAGCAGGTCGAGAGCCTGCTGCTCTCCACCCTCGAACCGGAGGCCGAGGTCGAGGCCTTCGAGC
AGGCGGTGGCGCAGCTCCCGGCCCCTCCCTGCGGACCGCGCATCGAACAATCGCTGATCAGCGGCAACCTGCTGCGCAGC
GAAGCGGTGACCCCGGCGCAGCGCCTCGCCGCGCTGGCCCAGCATGCCTGCGACGGCGCCAGCCACTGGTGGGGACGCGG
CTCGGCACGGATCTCCGCCGGCCTGATGCGCTACCAGGGCCTGCCGCCGGCGCCGGCGTTCGGACGATTCCTCACCGGAG
AGGGCGAGGTGATCCCGCTCTTTCCCGGCATACCCGGTTAA
TTGAACAGCGTCGGTTTCTACGGCAAGCTGGCCGGCCGCGGCGATTTCGTCAGCCGCGGCCTGCCGAACACCTTCGTCGA
ACCCTGGGACGCCTGGCTGGCCAGCGGCATGCGCGCCAGCCAGGACGAACTCGGCGCGGCCTGGCTCGACGCCTACCTGA
CCAGCCCGCTGTGGCGCTTCGCCATCGCCCCCGGCCTGCTCGGCGGCGAAGCGGTGGCCGGGGTGGTGATGCCGAGCATC
GACCGGGTCGGTCGCTATTTCCCGCTGACCGTCGCCTGCCTGCTGCCGGCGAACGCCGACCTCGGCGGGCTGGTCGGCGG
CGACGACGGCTGGTTCGAGCAGGTCGAGAGCCTGCTGCTCTCCACCCTCGAACCGGAGGCCGAGGTCGAGGCCTTCGAGC
AGGCGGTGGCGCAGCTCCCGGCCCCTCCCTGCGGACCGCGCATCGAACAATCGCTGATCAGCGGCAACCTGCTGCGCAGC
GAAGCGGTGACCCCGGCGCAGCGCCTCGCCGCGCTGGCCCAGCATGCCTGCGACGGCGCCAGCCACTGGTGGGGACGCGG
CTCGGCACGGATCTCCGCCGGCCTGATGCGCTACCAGGGCCTGCCGCCGGCGCCGGCGTTCGGACGATTCCTCACCGGAG
AGGGCGAGGTGATCCCGCTCTTTCCCGGCATACCCGGTTAA